BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

Semrock Part Number: DAPI-1160B-OFF-ZERO

IDEX Part Number: S-000166  /

Semrock
Highest-contrast "no burn-out" single-band filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP
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This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
7-hydroxy-4-methylcoumarin 360 442
Alexa Fluor® 350 343 441
Alexa Fluor® 405 401 422
AMCA (Aminomethylcoumarin) 350 448
ATTO 390 392 477
BD Horizon V450 406 449
BFP (EBFP) 380 440
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
DAPI 359 461
DEAC 432 472
DiFMU-pH 9.0 358 450
DY-415 418 470
DyLight 405 398 420
ECFP 435 475
HiLyte Fluor™ 405 403 428
Hoechst 33258 352 455
Hoechst 33342 352 455
Hoechst 34580 392 440
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
LysoTracker Blue 373 422
Marina Blue 363 461
mBBr+GSH 394 490
Pacific Blue™ 404 455
sgBFP™ 387 451
Sirius 355 424
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

OFF Holder/Cube

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63