BrightLine^® single-band filter set, optimized for DAPI and other like fluorophores

Semrock Part Number: DAPI-1160B-AMF-ZERO

IDEX Part Number: S-001399 /

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
1,8-ANS	372	477
4-methylumbelliferone	357	441
7-hydroxy-4-methylcoumarin	360	442
Alexa Fluor^® 350	343	441
Alexa Fluor^® 405	401	422
AMCA (Aminomethylcoumarin)	350	448
ATTO 390	392	477
BD Horizon V450	406	449
BFP (EBFP)	380	440
Calcofluor White	351	444
Cascade Blue™	401	419
CellTrace calcein violet	400	452
DAPI	359	461
DEAC	432	472
DiFMU-pH 9.0	358	450
DY-415	418	470
DyLight 405	398	420
ECFP	435	475
HiLyte Fluor™ 405	403	428
Hoechst 33258	352	455
Hoechst 33342	352	455
Hoechst 34580	392	440
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
LysoSensor Blue	374	424
LysoTracker Blue	373	422
Marina Blue	363	461
mBBr+GSH	394	490
Pacific Blue™	404	455
sgBFP™	387	451
Sirius	355	424
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437

Fluorophore