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BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

Semrock Part Number: DAPI-1160B-000

IDEX Part Number: S-000729  /

Semrock

Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving exceptionally low noise (background) yet high brightness, resulting in unsurpassed contrast (signal-to-noise ratio). Images of typical cell samples with DAPI-stained nuclei exhibit more than twice the contrast of those made with the leading competitor's set. The carefully optimized narrow, red-shifted exciter filter minimizes fluorophore photobleaching and sample phototoxicity. What's more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not "burn-out" under the intense UV illumination of modern fluorescence light sources.

This set is the unparalleled choice for most situations, especially those involving digital imaging. For specialized applications where brightness trumps contrast, use our DAPI-5060C set, which has the highest brightness on the market.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,055.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FF01-387/11-25

FL-004663

Each
  • Tavg > 90% 382 – 393 nm
  • Center Wavelength of 387.5 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FL-004663

FF02-447/60-25

FL-004651

Each
  • Tavg > 93% 417 – 477 nm
  • Center Wavelength of 447 nm and GMBW of 60 nm
  • FWHM of 65.3 nm

FL-004651

FF409-Di03-25x36

FL-007109

Each
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

FL-007109

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

DAPI-1160B-U03-ZERO

S-000737 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
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DAPI-1160B-U03

S-001099 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
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DAPI-5060C-000

S-000624 / 0

Out of Stock Backordered In Stock
Highest-brightness "no burn-out" single-band filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP
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DAPI-11LP-A-000

S-000738 / 0

Out of Stock Backordered In Stock
High contrast long-pass filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP
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DAPI-50LP-A-000

S-000634 / 0

Out of Stock Backordered In Stock
High brightness long-pass filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP
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GFP-3035D-000

S-000790 / 0

Out of Stock Backordered In Stock
High brightness and contrast single-band filter set. Ideal for use with: GFP , Cy2, CyQuant Cell Proliferation Assay, DiO, EGFP, sgGFP , wtGFP , YOYO-1, YO-PRO-1
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FITC-3540C-000

S-000752 / 0

Out of Stock Backordered In Stock
High brightness and contrast single-band filter set. Ideal for use with: FITC , 5-FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green, Oregon Green, Rhodamine 110, rsGFP
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TRITC-B-000

S-000957 / 0

Out of Stock Backordered In Stock
High brightness and contrast single-band filter set. Ideal for use with: TRITC , Alexa Fluor 546, 5-TAMRA , Alexa Fluor 532, SpectrumOrange, BODIPY TMR-X, DiI, POPO-3, PO-PRO-3, Rhodamine
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