This 5-filter "Pinkel" set is based on the award-winning BrightLine blue-green-red multiband filter set, but instead of a multiband exciter it has three single-band exciters, a multiband beamsplitter, and a multiband emitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore imaging using a low-noise monochrome camera.
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FF03-575/25-25
FL-004522 / 0
- Tavg > 93% 563 – 588 nm
- Center Wavelength of 575 nm and GMBW of 25 nm
- FWHM of 31.3 nm
FF01-457/530/628-25
FL-004534 / 0
- Tavg > 80% 446 – 468 nm, Tavg > 85% 520 – 540 nm & Tavg > 85% 614 – 642 nm
- Center Wavelengths of 457 nm, 530 nm & 628 nm and GMBWs of 22 nm, 20 nm & 28 nm
FF01-494/20-25
FL-004541 / 0
- Tavg > 93% 484 – 504 nm
- Center Wavelength of 494 nm and GMBW of 20 nm
- FWHM of 25.2 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF436/514/604-Di01-25x36
FL-007090 / 0
- Dichroic Beamsplitter with edges at 436 nm, 514 nm & 604 nm
- Ravg > 97.5% 394 – 414 nm, Ravg > 97.5% 484 – 504 nm & Ravg > 97.5% 566 – 586 nm
- Tavg > 90% 446 – 468 nm, Tavg > 90% 520 – 540 nm & Tavg > 90% 614 – 642 nm
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 4-methylumbelliferone | 357 | 441 |
|
|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
|
| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
|
| 6-carboxyrhodamine 6G | 525 | 555 |
|
|
| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Alexa Fluor® 405 | 401 | 422 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
|
| Alexa Fluor® 514 | 518 | 543 |
|
|
| Alexa Fluor® 568 | 579 | 603 |
|
|
| Alexa Fluor® 594 | 590 | 618 |
|
|
| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
|
| AsRed 2 | 576 | 592 |
|
|
| ATTO 390 | 392 | 477 |
|
|
| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| ATTO 565 | 563 | 592 |
|
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| ATTO 590 | 594 | 624 |
|
|
| ATTO 594 | 601 | 624 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BD Horizon V450 | 406 | 449 |
|
|
| BFP (EBFP) | 380 | 440 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY FL | 505 | 512 |
|
|
| BODIPY FL-MeOH | 502 | 511 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Crimson | 589 | 609 |
|
|
| Calcium Green-1 | 507 | 529 |
|
|
| Calcofluor White | 351 | 444 |
|
|
| Cascade Blue™ | 401 | 419 |
|
|
| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
|
| CellTrace calcein violet | 400 | 452 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
|
| CFP2 | 490 | 510 |
|
|
| Cy2™ | 492 | 507 |
|
|
| Cy3.5™ | 578 | 591 |
|
|
| CyQUANT GR-DNA | 502 | 523 |
|
|
| DAF-FM-NO | 495 | 519 |
|
|
| DAPI | 359 | 461 |
|
|
| Dendra2 (Green) | 491 | 507 |
|
|
| DiFMU-pH 9.0 | 358 | 450 |
|
|
| DiO | 475 | 500 |
|
|
| Dronpa | 502 | 516 |
|
|
| DsRed | 559 | 583 |
|
|
| dTomato | 556 | 582 |
|
|
| DY-505-Phalloidin | 503 | 530 |
|
|
| DY-590 | 582 | 599 |
|
|
| DyLight 405 | 398 | 420 |
|
|
| DyLight 594 | 593 | 618 |
|
|
| Emerald | 491 | 511 |
|
|
| ER-Tracker™ Blue-White DPX | 372 | 557 |
|
|
| FITC (Fluorescein) | 495 | 519 |
|
|
| FlAsH-CCPFCC | 511 | 530 |
|
|
| Fluo-3 | 506 | 527 |
|
|
| Fluo-4 | 494 | 516 |
|
|
| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
|
| Fluoro-Emerald | 494 | 518 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| GFP (EGFP) | 489 | 511 |
|
|
| Green 496 | 496 | 520 |
|
|
| Green 500 | 501 | 524 |
|
|
| HcRed1 | 588 | 618 |
|
|
| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
|
| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
|
| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
|
| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
|
| HiLyte Fluor™ 405 | 403 | 428 |
|
|
| HiLyte Fluor™ 488 | 497 | 526 |
|
|
| HiLyte Fluor™ 594 | 592 | 616 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
|
| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
|
| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| LysoTracker Green | 501 | 509 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| LysoTracker Yellow HCK-123 | 488 | 565 |
|
|
| Magnesium Green | 507 | 531 |
|
|
| mApple | 566 | 594 |
|
|
| Marina Blue | 363 | 461 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| mCherry | 587 | 610 |
|
|
| mHoneyDew | 478 | 562 |
|
|
| MitoTracker™ Green | 490 | 512 |
|
|
| MitoTracker™ Red | 578 | 598 |
|
|
| mKate2 | 588 | 633 |
|
|
| mPlum | 589 | 649 |
|
|
| mRFP1 | 584 | 700 |
|
|
| mRuby | 558 | 605 |
|
|
| mStrawberry | 574 | 596 |
|
|
| mTangerine | 568 | 585 |
|
|
| mWasabi | 493 | 509 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Nile red-phospolipid | 553 | 637 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
|
| Oregon Green™ 514 | 513 | 532 |
|
|
| Pacific Blue™ | 404 | 455 |
|
|
| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| PicoGreen® | 502 | 522 |
|
|
| PKH67 | 488 | 500 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine 123 | 507 | 529 |
|
|
| Rhodamine Green | 497 | 523 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
|
| Sodium Green | 507 | 532 |
|
|
| SpectrumBlue | 405 | 449 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SpectrumRed | 587 | 615 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA |