This 7-filter "Sedat" set is based on the award-winning BrightLine blue-green-red multiband filter set, but instead of multiband exciter and emitter filters, it has three single-band exciters, three single-band emitters, and a triple-band beamsplitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore high-speed sequential imaging using a low-noise monochrome camera and filter wheels for both the exciters and the emitters.
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FF02-531/22-25
FL-004200 / 0
- Tavg > 93% 519.5 – 542 nm
- Center Wavelength of 530.8 nm and GMBW of 22.5 nm
- FWHM of 28.1 nm
FF01-624/40-25
FL-004395 / 0
- Tavg > 93% 604 – 644 nm
- Center Wavelength of 624 nm and GMBW of 40 nm
- FWHM of 45.8 nm
FF03-575/25-25
FL-004522 / 0
- Tavg > 93% 563 – 588 nm
- Center Wavelength of 575 nm and GMBW of 25 nm
- FWHM of 31.3 nm
FF01-494/20-25
FL-004541 / 0
- Tavg > 93% 484 – 504 nm
- Center Wavelength of 494 nm and GMBW of 20 nm
- FWHM of 25.2 nm
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF436/514/604-Di01-25x36
FL-007090 / 0
- Dichroic Beamsplitter with edges at 436 nm, 514 nm & 604 nm
- Ravg > 97.5% 394 – 414 nm, Ravg > 97.5% 484 – 504 nm & Ravg > 97.5% 566 – 586 nm
- Tavg > 90% 446 – 468 nm, Tavg > 90% 520 – 540 nm & Tavg > 90% 614 – 642 nm
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 2-dodecylresorufin-lipid | 582 | 595 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 514 | 518 | 543 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| AsRed 2 | 576 | 592 |
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| ATTO 390 | 392 | 477 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 565 | 563 | 592 |
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| ATTO 590 | 594 | 624 |
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