BrightLine® Sedat filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

Semrock Part Number: DA/FI/TX-3X3M-C-ZHE

IDEX Part Number: S-001392  /

Semrock

This 7-filter "Sedat" set is based on the award-winning BrightLine blue-green-red multiband filter set, but instead of multiband exciter and emitter filters, it has three single-band exciters, three single-band emitters, and a triple-band beamsplitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore high-speed sequential imaging using a low-noise monochrome camera and filter wheels for both the exciters and the emitters.

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FF02-531/22-25

FL-004200 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 519.5 – 542 nm
  • Center Wavelength of 530.8 nm and GMBW of 22.5 nm
  • FWHM of 28.1 nm

FF01-624/40-25

FL-004395 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 604 – 644 nm
  • Center Wavelength of 624 nm and GMBW of 40 nm
  • FWHM of 45.8 nm

FF03-575/25-25

FL-004522 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 563 – 588 nm
  • Center Wavelength of 575 nm and GMBW of 25 nm
  • FWHM of 31.3 nm

FF01-494/20-25

FL-004541 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 484 – 504 nm
  • Center Wavelength of 494 nm and GMBW of 20 nm
  • FWHM of 25.2 nm

FF02-447/60-25

FL-004651 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 417 – 477 nm
  • Center Wavelength of 447 nm and GMBW of 60 nm
  • FWHM of 65.3 nm

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF436/514/604-Di01-25x36

FL-007090 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edges at 436 nm, 514 nm & 604 nm
  • Ravg > 97.5% 394 – 414 nm, Ravg > 97.5% 484 – 504 nm & Ravg > 97.5% 566 – 586 nm
  • Tavg > 90% 446 – 468 nm, Tavg > 90% 520 – 540 nm & Tavg > 90% 614 – 642 nm

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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
6-carboxyrhodamine 6G 525 555
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AMCA (Aminomethylcoumarin) 350 448
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 390 392 477
ATTO 488 500 525
ATTO 495 495 527
ATTO 565 563 592
ATTO 590 594 624
ATTO 594 601