BrightLine® Sedat filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

Semrock Part Number: DA/FI/TX-3X3M-C-LDMK

IDEX Part Number: S-001387  /

Semrock

This 7-filter "Sedat" set is based on the award-winning BrightLine blue-green-red multiband filter set, but instead of multiband exciter and emitter filters, it has three single-band exciters, three single-band emitters, and a triple-band beamsplitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore high-speed sequential imaging using a low-noise monochrome camera and filter wheels for both the exciters and the emitters.

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  • Price Not Available.
  • 3,194.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
6-carboxyrhodamine 6G 525 555
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AMCA (Aminomethylcoumarin) 350 448
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 390 392 477
ATTO 488 500 525
ATTO 495 495 527
ATTO 565 563 592
ATTO 590 594 624
ATTO 594 601 624
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-3 570 605
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace BODIPY TR methyl ester 598 625
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
DEAC 432 472
Dendra2 (Green) 491 507
DiFMU-pH 9.0 358 450
DiO 475 500
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-415 418 470
DY-505-Phalloidin 503 530
DY-590 582 599
DyLight 405 398 420
DyLight 594 593 618
ECFP 435 475
Emerald 491 511
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 405 403 428
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
Hoechst 33258 352 455
Hoechst 33342 352 455
Hoechst 34580 392 440
Hypericin 600 603
KFP-Red 575 599
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
LysoTracker Blue 373 422
LysoTracker Green 501 509
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mApple 566 594
Marina Blue 363 461
mBBr+GSH 394 490
mCherry 587 610
mHoneyDew 478 562
MitoTracker™ Green 490 512
MitoTracker™ Red 578 598
mKate2 588 633
mPlum 589 649
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile red-phospolipid 553 637
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
Pacific Blue™ 404 455
pHrodo™, succinimidyl ester 560 587
PicoGreen® 502 522
PKH67 488 500
Propidium Iodide (PI) 305 617
Qdot® 525 Nanocrystals 300 525
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
ReAsH-CCPGCC 592 606
Red 580 580 603
Resorufin 571 584
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodamine Red-X 573 591
Rhodol Green 497 524
sgBFP™ 387 451
Sirius 355 424
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
Sodium Green 507 532
SpectrumBlue 405 449
SpectrumGreen 497 538
SpectrumOrange 554 587
SpectrumRed 587 615
Sulforhodamine 101-EtOH 578 593
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 17 619 638
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TagBFP 402 457
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TO-PRO-1 515 531
TOTO-1 514 531
TurboFP635(Katushka) 591 638
TurboGFP 482 503
Venus 516 528
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
Vybrant DyeCycle Violet 369 437
X-Rhod-1 Indicator 580 601
YFP (yellow GFP) 513 530
YO-PRO-1 491 506
YO-PRO-3 613 629
YOYO-1 491 508
YOYO-3 611 631

LDMK Holder/Cube

This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm