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BrightLine® Sedat filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

Semrock Part Number: DA/FI/TX-3X3M-C-000

IDEX Part Number: S-000622  /

Semrock

This 7-filter "Sedat" set is based on the award-winning BrightLine blue-green-red multiband filter set, but instead of multiband exciter and emitter filters, it has three single-band exciters, three single-band emitters, and a triple-band beamsplitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore high-speed sequential imaging using a low-noise monochrome camera and filter wheels for both the exciters and the emitters.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 2,805.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FF01-387/11-25

FL-004663

Each
  • Tavg > 90% 382 – 393 nm
  • Center Wavelength of 387.5 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FL-004663

FF01-494/20-25

FL-004541

Each
  • Tavg > 93% 484 – 504 nm
  • Center Wavelength of 494 nm and GMBW of 20 nm
  • FWHM of 25.2 nm

FL-004541

FF01-624/40-25

FL-004395

Each
  • Tavg > 93% 604 – 644 nm
  • Center Wavelength of 624 nm and GMBW of 40 nm
  • FWHM of 45.8 nm

FL-004395

FF02-447/60-25

FL-004651

Each
  • Tavg > 93% 417 – 477 nm
  • Center Wavelength of 447 nm and GMBW of 60 nm
  • FWHM of 65.3 nm

FL-004651

FF02-531/22-25

FL-004200

Each
  • Tavg > 93% 519.5 – 542 nm
  • Center Wavelength of 530.8 nm and GMBW of 22.5 nm
  • FWHM of 28.1 nm

FL-004200

FF03-575/25-25

FL-004522

Each
  • Tavg > 93% 563 – 588 nm
  • Center Wavelength of 575 nm and GMBW of 25 nm
  • FWHM of 31.3 nm

FL-004522

FF436/514/604-Di01-25x36

FL-007090

Each
  • Dichroic Beamsplitter with edges at 436 nm, 514 nm & 604 nm
  • Ravg > 97.5% 394 – 414 nm, Ravg > 97.5% 484 – 504 nm & Ravg > 97.5% 566 – 586 nm
  • Tavg > 90% 446 – 468 nm, Tavg > 90% 520 – 540 nm & Tavg > 90% 614 – 642 nm

FL-007090

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

DA/FI/TX-3X3M-C-NQF

S-001389 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

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DA/FI/TX-3X3M-C-NTE

S-001390 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

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DA/FI/TX-3X3M-C-OMF

S-000151 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

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DA/FI/TX-3X3M-C-ZHE

S-001392 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

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DA/FI/TX-3X3M-C-OFF

S-001391 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
6-carboxyrhodamine 6G 525 555
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AMCA (Aminomethylcoumarin) 350 448
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 390 392 477
ATTO 488 500 525
ATTO 495 495 527
ATTO 565 563 592
ATTO 590 594 624
ATTO 594 601 624
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-3 570 605
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace BODIPY TR methyl ester 598 625
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
DEAC 432 472
Dendra2 (Green) 491 507
DiFMU-pH 9.0 358 450
DiO 475 500
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-415 418 470
DY-505-Phalloidin 503 530
DY-590 582 599
DyLight 405 398 420
DyLight 594 593 618
ECFP 435 475
Emerald 491 511
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 405 403 428
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
Hoechst 33258 352 455