This 7-filter "Pinkel" penta-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, Cy5, and Cy7 (and other key fluorophores – see complete list above). The complete set is comprised of a penta-band beamsplitter and emission filter and five single-band exciters – all "no burn-out". The single-band filters are intended to be mounted in filter wheels.
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This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||25.0 mm||5.0 mm|
|Emitter (Diameter)||25.0 mm||5.0 mm|
|Dichroic (L × W)||25.2 mm × 35.6 mm||1.5 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|
|What is Pixel Shift, and how does the Semrock ZERO™ option help?||Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.|
|Fluorophore||Peak Excitation Wavelength(nm)||Peak Emission Wavelength(nm)||Compatibility||Display on Plot above|
|5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)||542||568||
|7-AAD (7-aminoactinomycin D)||548||648||
|Acridine Orange (+DNA)||502||526||
|Alexa Fluor® 405||401||422||
|Alexa Fluor® 488||499||520||
|Alexa Fluor® 500||503||525||