This 7-filter "Pinkel" penta-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, Cy5, and Cy7 (and other key fluorophores – see complete list above). The complete set is comprised of a penta-band beamsplitter and emission filter and five single-band exciters – all "no burn-out". The single-band filters are intended to be mounted in filter wheels.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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3,904.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-740/13-25
FL-004290 / 0
- Tavg > 93% 733.5 – 746.5 nm
- Center Wavelength of 740 nm and GMBW of 13 nm
- FWHM of 19.9 nm
FF01-650/13-25
FL-004397 / 0
- Tavg > 93% 643.5 – 656.5 nm
- Center Wavelength of 650 nm and GMBW of 13 nm
- FWHM of 20.1 nm
FF02-485/20-25
FL-004432 / 0
- Tavg > 93% 475 – 495 nm
- Center Wavelength of 485 nm and GMBW of 20 nm
- FWHM of 24.9 nm
FF01-440/521/607/694/809-25
FL-004444 / 0
- Tavg > 90% 420 – 460 nm, Tavg > 90% 510 – 531 nm, Tavg > 90% 589.5 – 623.5 nm, Tavg > 90% 677 – 711.5 nm & Tavg > 90% 768.5 – 849.5 nm
- Center Wavelengths of 440 nm, 520.5 nm, 606.5 nm, 694.3 nm & 809 nm and GMBWs of 40 nm, 21 nm, 34 nm, 34.5 nm & 81 nm
FF01-560/25-25
FL-004490 / 0
- Tavg > 93% 547 – 572 nm
- Center Wavelength of 559.5 nm and GMBW of 25 nm
- FWHM of 32.3 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 381.5 – 392.5 nm
- Center Wavelength of 387 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF408/504/581/667/762-Di01-25x36
FL-007009 / 0
- Dichroic Beamsplitter with edges at 408 nm, 504 nm, 581 nm, 667 nm & 762 nm
- Ravg > 95% 381.5 – 392.5 nm, Ravg > 95% 475 – 495 nm, Ravg > 95% 547 – 572 nm, Ravg > 95% 643 – 656 nm & Ravg > 95% 733.5 – 746.5 nm
- Tavg > 90% 420 – 460 nm, Tavg > 90% 510 – 531 nm, Tavg > 90% 589.5 – 623.5 nm, Tavg > 90% 677 – 711.5 nm & Tavg > 90% 768.5 – 849.5 nm
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This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 2-dodecylresorufin-lipid | 582 | 595 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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