This 7-filter "Pinkel" penta-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, Cy5, and Cy7 (and other key fluorophores – see complete list above). The complete set is comprised of a penta-band beamsplitter and emission filter and five single-band exciters – all "no burn-out". The single-band filters are intended to be mounted in filter wheels.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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3,854.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-740/13-25
FL-004290 / 0
- Tavg > 93% 733.5 – 746.5 nm
- Center Wavelength of 740 nm and GMBW of 13 nm
- FWHM of 19.9 nm
FF01-650/13-25
FL-004397 / 0
- Tavg > 93% 643.5 – 656.5 nm
- Center Wavelength of 650 nm and GMBW of 13 nm
- FWHM of 20.1 nm
FF02-485/20-25
FL-004432 / 0
- Tavg > 93% 475 – 495 nm
- Center Wavelength of 485 nm and GMBW of 20 nm
- FWHM of 24.9 nm
FF01-440/521/607/694/809-25
FL-004444 / 0
- Tavg > 90% 420 – 460 nm, Tavg > 90% 510 – 531 nm, Tavg > 90% 589.5 – 623.5 nm, Tavg > 90% 677 – 711.5 nm & Tavg > 90% 768.5 – 849.5 nm
- Center Wavelengths of 440 nm, 520.5 nm, 606.5 nm, 694.3 nm & 809 nm and GMBWs of 40 nm, 21 nm, 34 nm, 34.5 nm & 81 nm
FF01-560/25-25
FL-004490 / 0
- Tavg > 93% 547 – 572 nm
- Center Wavelength of 559.5 nm and GMBW of 25 nm
- FWHM of 32.3 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF408/504/581/667/762-Di01-25x36
FL-007009 / 0
- Dichroic Beamsplitter with edges at 408 nm, 504 nm, 581 nm, 667 nm & 762 nm
- Ravg > 95% 381.5 – 392.5 nm, Ravg > 95% 475 – 495 nm, Ravg > 95% 547 – 572 nm, Ravg > 95% 643 – 656 nm & Ravg > 95% 733.5 – 746.5 nm
- Tavg > 90% 420 – 460 nm, Tavg > 90% 510 – 531 nm, Tavg > 90% 589.5 – 623.5 nm, Tavg > 90% 677 – 711.5 nm & Tavg > 90% 768.5 – 849.5 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 2-dodecylresorufin-lipid | 582 | 595 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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