BrightLine® Pinkel filter set, optimized for DAPI, FITC, TRITC, Cy5 & Cy7 and other like fluorophores

Semrock Part Number: DA/FI/TR/Cy5/Cy7-5X-A-NQF

IDEX Part Number: S-001371  /

Semrock

This 7-filter "Pinkel" penta-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, Cy5, and Cy7 (and other key fluorophores – see complete list above). The complete set is comprised of a penta-band beamsplitter and emission filter and five single-band exciters – all "no burn-out". The single-band filters are intended to be mounted in filter wheels.

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  • 3,854.00 USD Each
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FF01-740/13-25

FL-004290 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 733.5 – 746.5 nm
  • Center Wavelength of 740 nm and GMBW of 13 nm
  • FWHM of 19.9 nm

FF01-650/13-25

FL-004397 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 643.5 – 656.5 nm
  • Center Wavelength of 650 nm and GMBW of 13 nm
  • FWHM of 20.1 nm

FF02-485/20-25

FL-004432 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 475 – 495 nm
  • Center Wavelength of 485 nm and GMBW of 20 nm
  • FWHM of 24.9 nm

FF01-440/521/607/694/809-25

FL-004444 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 420 – 460 nm, Tavg > 90% 510 – 531 nm, Tavg > 90% 589.5 – 623.5 nm, Tavg > 90% 677 – 711.5 nm & Tavg > 90% 768.5 – 849.5 nm
  • Center Wavelengths of 440 nm, 520.5 nm, 606.5 nm, 694.3 nm & 809 nm and GMBWs of 40 nm, 21 nm, 34 nm, 34.5 nm & 81 nm

FF01-560/25-25

FL-004490 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 547 – 572 nm
  • Center Wavelength of 559.5 nm and GMBW of 25 nm
  • FWHM of 32.3 nm

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF408/504/581/667/762-Di01-25x36

FL-007009 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edges at 408 nm, 504 nm, 581 nm, 667 nm & 762 nm
  • Ravg > 95% 381.5 – 392.5 nm, Ravg > 95% 475 – 495 nm, Ravg > 95% 547 – 572 nm, Ravg > 95% 643 – 656 nm & Ravg > 95% 733.5 – 746.5 nm
  • Tavg > 90% 420 – 460 nm, Tavg > 90% 510 – 531 nm, Tavg > 90% 589.5 – 623.5 nm, Tavg > 90% 677 – 711.5 nm & Tavg > 90% 768.5 – 849.5 nm

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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm

1.1 mm
(Can accept a 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578