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BrightLine® Sedat filter set, optimized for DAPI, FITC, TRITC, Cy5 & Cy7 and other like fluorophores

Semrock Part Number: DA/FI/TR/Cy5/Cy7-5X5M-B-000

IDEX Part Number: S-000619  /

Semrock

This 11-filter "Sedat" penta-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, Cy5, and Cy7 (and other key fluorophores – see complete list above). The complete set is comprised of a penta-band beamsplitter with five single-band emitters and five single-band exciters – all "no burn-out". The single-band filters are intended to be mounted in filter wheels.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 4,620.75 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
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Set Components

FF01-387/11-25

FL-004663

Each
  • Tavg > 90% 382 – 393 nm
  • Center Wavelength of 387.5 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FL-004663

FF01-440/40-25

FL-004590

Each
  • Tavg > 93% 420 – 460 nm
  • Center Wavelength of 440 nm and GMBW of 40 nm
  • FWHM of 45.6 nm

FL-004590

FF01-525/30-25

FL-004450

Each
  • Tavg > 90% 510 – 540 nm
  • Center Wavelength of 525 nm and GMBW of 30 nm
  • FWHM of 35.8 nm

FL-004450

FF01-560/25-25

FL-004490

Each
  • Tavg > 93% 547 – 572 nm
  • Center Wavelength of 559.5 nm and GMBW of 25 nm
  • FWHM of 32.3 nm

FL-004490

FF01-607/36-25

FL-004110

Each
  • Tavg > 93% 589 – 625 nm
  • Center Wavelength of 607 nm and GMBW of 36 nm
  • FWHM of 42.5 nm

FL-004110

FF01-650/13-25

FL-004397

Each
  • Tavg > 93% 643.5 – 656.5 nm
  • Center Wavelength of 650 nm and GMBW of 13 nm
  • FWHM of 20.1 nm

FL-004397

FF01-740/13-25

FL-004290

Each
  • Tavg > 93% 733.5 – 746.5 nm
  • Center Wavelength of 740 nm and GMBW of 13 nm
  • FWHM of 19.9 nm

FL-004290

FF02-485/20-25

FL-004432

Each
  • Tavg > 93% 475 – 495 nm
  • Center Wavelength of 485 nm and GMBW of 20 nm
  • FWHM of 24.9 nm

FL-004432

FF02-684/24-25

FL-004574

Each
  • Tavg > 93% 672 – 696 nm
  • Center Wavelength of 684 nm and GMBW of 24 nm
  • FWHM of 30.1 nm

FL-004574

FF02-809/81-25

FL-004520

Each
  • Tavg > 93% 768.5 – 849.5 nm
  • Center Wavelength of 809 nm and GMBW of 81 nm
  • FWHM of 88.4 nm

FL-004520

FF408/504/581/667/762-Di01-25x36

FL-007009

Each
  • Dichroic Beamsplitter with edges at 408 nm, 504 nm, 581 nm, 667 nm & 762 nm
  • Ravg > 95% 381.5 – 392.5 nm, Ravg > 95% 475 – 495 nm, Ravg > 95% 547 – 572 nm, Ravg > 95% 643 – 656 nm & Ravg > 95% 733.5 – 746.5 nm
  • Tavg > 90% 420 – 460 nm, Tavg > 90% 510 – 531 nm, Tavg > 90% 589.5 – 623.5 nm, Tavg > 90% 677 – 711.5 nm & Tavg > 90% 768.5 – 849.5 nm

FL-007009

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

DA/FI/TR/Cy5/Cy7-5X5M-B-U03

S-422605 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
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DA/FI/TR/Cy5/Cy7-5X5M-B-NQF

S-001379 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

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More Info
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DA/FI/TR/Cy5/Cy7-5X5M-B-NTE

S-000122 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

Compare Compare
More Info
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DA/FI/TR/Cy5/Cy7-5X5M-B-OMF

S-000125 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

Compare Compare
More Info
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DA/FI/TR/Cy5/Cy7-5X5M-B-ZHE

S-001380 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

Compare Compare
More Info
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DA/FI/TR/Cy5/Cy7-5X5M-B-OFF

S-000124 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
Alexa Fluor® 750 751 776
Alexa Fluor® 790 782 805
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 488 500 525
ATTO 495 495 527
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
ATTO 725 727 754
ATTO 740 740 764
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-3 570 605
BODIPY 650/665-X 646 664
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Calcium Orange 589 609
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace BODIPY TR methyl ester 598 625
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
Cy5.5™ 672 690
Cy5™ 649 666
Cy7™ 753 775
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
DDAO 648 657
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DiFMU-pH 9.0 358 450
DiI 551 569
DiO 475 500
DiR 750 782
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-505-Phalloidin 503 530
DY-590 582 599
DY-630 634 656
DY-675 675 699
DY-781 784 802
DyLight 405 398 420
DyLight 594 593 618
DyLight 649 651 673
DyLight 680 676 705
DyLight 750 751 778
DyLight 800 777 794
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Dark Red fluorescent microspheres 656 683
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 405 403 428
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
HiLyte Fluor™ 647 649 673
HiLyte Fluor™ 750 750 778
IRDye 800 CW-MeOH 778 801
IRDye 800CW 775 792
IRDye 800CW-NHS Ester 775 792
IRDye 800RS 787 812
KFP-Red 575 599
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LIVE-DEAD Fixable Near-IR Dead Cell Stain 752 776
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LOLO-1 568 580
LysoSensor Blue 374 424
LysoSensor Green 448 502
LysoTracker Blue 373 422
LysoTracker Green 501 509
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
Magnesium Orange 550 574
mApple 566 594
Marina Blue 363 461
mCherry 587 610
Merocyanine 540 559 579
mHoneyDew 478 562
MitoTracker™ Deep Red 641 662
MitoTracker™ Green 490 512
MitoTracker™ Orange 551 575
MitoTracker™ Red 578 598
mKate2 588 633
mOrange 546 562
mOrange2 551 565
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile blue-EtOH 631 660
Nile red-phospolipid 553 637
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
Pacific Blue™ 404 455
pHrodo™, succinimidyl ester 560 587
PicoGreen® 502 522
PKH67 488 500
POPO-3 533 574
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 525 Nanocrystals 300 525
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 800 Nanocrystals 300 792
ratiometric pHluorin pH5 500 509
ReAsH-CCPGCC 592 606
Red 580 580 603
Red 650 649 665
Resorufin 571 584
Rhod-2 553 577
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
Rhodol Green 497 524
sgBFP™ 387 451
sgGFP™ (super glow GFP) 472 506
Sirius 355 424
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
Sodium Green 507 532
SpectrumBlue 405 449
SpectrumFRed (Far Red) 650 673
SpectrumGreen 497 538
SpectrumOrange 554 587
Sulforhodamine 101-EtOH 578 593
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 60 650 681
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
SYTOX Orange 547 570
SYTOX Red-DNA 640 658
TagBFP 402 457
Tetramethylrhodamine dextran 554 582
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TO-PRO-1 515 531
TO-PRO-3 642 657
TOTO-1 514 531
TOTO-3 642 661
TRITC (Tetramethylrhodamine) 552 578
TurboFP635(Katushka) 591 638
TurboGFP 482 503
TurboRFP 553 573
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Violet 369 437
wtGFP (wild type GFP, non-UV excitation) 474 509
X-Rhod-1 Indicator 580 601
YFP (yellow GFP) 513 530
YO-PRO-1 491 506
YOYO-1 491 508

Specifications

DA/FI/TR/Cy5/Cy7-5X5M-B-U03

S-422605 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
Compare Compare
More Info
Compare Compare

DA/FI/TR/Cy5/Cy7-5X5M-B-NQF

S-001379 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

Compare Compare
More Info
Compare Compare

DA/FI/TR/Cy5/Cy7-5X5M-B-NTE

S-000122 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

Compare Compare
More Info
Compare Compare

DA/FI/TR/Cy5/Cy7-5X5M-B-OMF

S-000125 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

Compare Compare
More Info
Compare Compare

DA/FI/TR/Cy5/Cy7-5X5M-B-ZHE

S-001380 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

Compare Compare
More Info
Compare Compare

DA/FI/TR/Cy5/Cy7-5X5M-B-OFF

S-000124 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

Compare Compare
More Info
Compare Compare
Hide Show
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/ 

1 of 1
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

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