This Full-multiband quad-band set is designed for imaging DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). The complete set is comprised of a quad-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,219.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-387/485/559/649-25
FL-004249 / 0
- Tavg > 85% 381.5 – 392.5 nm, Tavg > 90% 475 – 495 nm, Tavg > 90% 547 – 572 nm & Tavg> 90% 643 – 656 nm
- Center Wavelengths of 387 nm, 485 nm, 559.5 nm & 649.5 nm and GMBWs of 11 nm, 20 nm, 25 nm & 19.5 nm
FF01-440/521/607/700-25
FL-004445 / 0
- Tavg > 90% 420 – 460 nm, Tavg > 90% 510.5 – 531.5 nm, Tavg > 90% 590 – 624 nm & Tavg > 90% 677.5 – 722.5 nm
- Center Wavelengths of 440 nm, 521 nm, 607 nm & 700 nm and GMBWs of 40 nm, 21 nm, 34 nm & 45 nm
FF410/504/582/669-Di01-25x36
FL-007087 / 0
- Dichroic Beamsplitter with edges at 410 nm, 504 nm, 582 nm & 669 nm
- Ravg > 95% 381.5 – 392.5 nm, Ravg > 95% 475 – 495 nm, Ravg > 95% 547 – 572 nm & Ravg > 95% 643 – 656 nm
- Tavg > 90% 420 – 460 nm, Tavg > 90% 510 – 531 nm, Tavg > 90% 589.5 – 623.5 nm & Tavg > 90% 677 – 722 nm
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
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| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 4-methylumbelliferone | 357 | 441 |
|
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
|
| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
|
| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Alexa Fluor® 405 | 401 | 422 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
|
| Alexa Fluor® 546 | 556 | 572 |
|
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| Alexa Fluor® 555 | 553 | 565 |
|
|
| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 594 | 590 | 618 |
|
|
| Alexa Fluor® 647 | 653 | 668 |
|
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| Alexa Fluor® 660 | 663 | 691 |
|
|
| Alexa Fluor® 680 | 679 | 702 |
|
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| Alexa Fluor® 700 | 696 | 719 |
|
|
| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
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| AmCyan1 | 458 | 489 |
|
|
| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 590 | 594 | 624 |
|
|
| ATTO 635 | 635 | 658 |
|
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| ATTO 647 | 644 | 670 |
|
|
| ATTO 647N | 645 | 668 |
|
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| ATTO 655 | 663 | 683 |
|
|
| ATTO 680 | 680 | 700 |
|
|
| ATTO 700 | 699 | 719 |
|
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| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BD Horizon V450 | 406 | 449 |
|
|
| BFP (EBFP) | 380 | 440 |
|
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY 650/665-X | 646 | 664 |
|
|
| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
|
| BODIPY TMR-X | 544 | 573 |
|
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Crimson | 589 | 609 |
|
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| Calcium Green-1 | 507 | 529 |
|
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| Calcium Orange | 589 | 609 |
|
|
| Calcofluor White | 351 | 444 |
|
|
| Cascade Blue™ | 401 | 419 |
|
|
| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
|
| CellTrace calcein violet | 400 | 452 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
|
| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
|
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| Cy5.5™ | 672 | 690 |
|
|
| Cy5™ | 649 | 666 |
|
|
| CyQUANT GR-DNA | 502 | 523 |
|
|
| DAF-FM-NO | 495 | 519 |
|
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| DAPI | 359 | 461 |
|
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| Dendra2 (Green) | 491 | 507 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
|
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| DiFMU-pH 9.0 | 358 | 450 |
|
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| DiI | 551 | 569 |
|
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| DiO | 475 | 500 |
|
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| Dronpa | 502 | 516 |
|
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| DsRed | 559 | 583 |
|
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| DsRed-Express | 556 | 584 |
|
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| dTomato | 556 | 582 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
|
| DY-590 | 582 | 599 |
|
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| DY-675 | 675 | 699 |
|
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| DyLight 405 | 398 | 420 |
|
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| DyLight 594 | 593 | 618 |
|
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| DyLight 649 | 651 | 673 |
|
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| DyLight 680 | 676 | 705 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
|
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| Ethidium bromide | 518 | 603 |
|
|
| Ethidium homodimer | 527 | 617 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
|
| FlAsH-CCPFCC | 511 | 530 |
|
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| Fluo-3 | 506 | 527 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
|
| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
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| HcRed1 | 588 | 618 |
|
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| HiLyte Fluor™ 594 | 592 | 616 |
|
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| HiLyte Fluor™ 647 | 649 | 673 |
|
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| HiLyte Fluor™ 680 | 688 | 702 |
|
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| IRDye 680 NHS Ester | 683 | 710 |
|
|
| IRDye 700Phosphoramidite | 691 | 699 |
|
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| KFP-Red | 575 | 599 |
|
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
|
| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
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| LOLO-1 | 568 | 580 |
|
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| LysoSensor Blue | 374 | 424 |
|
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| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Blue | 373 | 422 |
|
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| LysoTracker Green | 501 | 509 |
|
|
| LysoTracker Red | 573 | 592 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Green | 507 | 531 |
|
|
| Magnesium Orange | 550 | 574 |
|
|
| mApple | 566 | 594 |
|
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| Marina Blue | 363 | 461 |
|
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| mCherry | 587 | 610 |
|
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| Merocyanine 540 | 559 | 579 |
|
|
| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Deep Red | 641 | 662 |
|
|
| MitoTracker™ Green | 490 | 512 |
|
|
| MitoTracker™ Orange | 551 | 575 |
|
|
| MitoTracker™ Red | 578 | 598 |
|
|
| mKate2 | 588 | 633 |
|
|
| mOrange | 546 | 562 |
|
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| mOrange2 | 551 | 565 |
|
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| mRFP | 555 | 583 |
|
|
| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
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| mWasabi | 493 | 509 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
|
| Nile red-phospolipid | 553 | 637 |
|
|
| Nile red-triglyceride | 510 | 583 |
|
|
| OFP | 547 | 567 |
|
|
| Orange 552 | 552 | 574 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
|
| Oregon Green™ 514 | 513 | 532 |
|
|
| Pacific Blue™ | 404 | 455 |
|
|
| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| PicoGreen® | 502 | 522 |
|
|
| PKH67 | 488 | 500 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Pro-Q Diamond | 556 | 583 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Quasar® 705 | 688 | 706 |
|
|
| ratiometric pHluorin pH5 | 500 | 509 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Red 650 | 649 | 665 |
|
|
| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine 123 | 507 | 529 |
|
|
| Rhodamine Green | 497 | 523 |
|
|
| Rhodamine phalloidin | 558 | 575 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| Sirius | 355 | 424 |
|
|
| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
|
| Sodium Green | 507 | 532 |
|
|
| SpectrumBlue | 405 | 449 |
|
|
| SpectrumFRed (Far Red) | 650 | 673 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
|
| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
|
| SYTO 11 | 506 | 525 |
|
|
| SYTO 13 | 448 | 506 |
|
|
| SYTO 16 | 489 | 520 |
|
|
| SYTO 60 | 650 | 681 |
|
|
| SYTO 9 | 483 | 500 |
|
|
| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| SYTOX Orange | 547 | 570 |
|
|
| SYTOX Red-DNA | 640 | 658 |
|
|
| TagBFP | 402 | 457 |
|
|
| Tetramethylrhodamine dextran | 554 | 582 |
|
|
| Texas Red dextran | 592 | 613 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| Texas Red® | 592 | 614 |
|
|
| TO-PRO-1 | 515 | 531 |
|
|
| TO-PRO-3 | 642 | 657 |
|
|
| TOTO-1 | 514 | 531 |
|
|
| TOTO-3 | 642 | 661 |
|
|
| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
|
| TurboFP635(Katushka) | 591 | 638 |
|
|
| TurboGFP | 482 | 503 |
|
|
| TurboRFP | 553 | 573 |
|
|
| Vybrant DyeCycle Green | 506 | 534 |
|
|
| Vybrant DyeCycle Violet | 369 | 437 |
|
|
| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| X-Rhod-1 Indicator | 580 | 601 |