This blue-green-orange multiband filter set is uniquely optimized to provide simultaneously stunning brightness, striking contrast, and superb color balance when viewing multiple fluorophores by eye or with a high-quality color CCD camera. The result is brilliant colors and a black background.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,999.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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Select all 3 products from all pages.
FF01-387/478/555-25
FL-004331 / 0
- Tavg > 80% 382 – 393 nm, Tavg > 90% 466 – 490 nm & Tavg > 90% 546 – 565 nm
- Center Wavelengths of 387.5 nm, 478 nm & 555.5 nm and GMBWs of 11 nm, 24 nm & 19 nm
FF01-433/517/613-25
FL-004334 / 0
- Tavg > 90% 414 – 452 nm, Tavg > 90% 505 – 530 nm & Tavg > 90% 582 – 645 nm
- Center Wavelengths of 433 nm, 517.5 nm & 613.5 nm and GMBWs of 38 nm, 25 nm & 63 nm
FF403/497/574-Di01-25x36
FL-007049 / 0
- Dichroic Beamsplitter with edges at 403 nm, 497 nm & 574 nm
- Ravg > 92% 370 – 393 nm, Ravg > 97.5% 466 – 490 nm & Ravg > 97.5% 546 – 565 nm
- Tavg > 90% 414 – 450 nm, Tavg > 90% 507.5 – 528 nm & Tavg > 90% 584 – 645 nm
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
|
| 4-methylumbelliferone | 357 | 441 |
|
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
|
| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
|
| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Acridine Yellow | 470 | 550 |
|
|
| Alexa Fluor® 405 | 401 | 422 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
|
| Alexa Fluor® 546 | 556 | 572 |
|
|
| Alexa Fluor® 555 | 553 | 565 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
|
| Alexa Fluor® 594 | 590 | 618 |
|
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
|
| AmCyan1 | 458 | 489 |
|
|
| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 590 | 594 | 624 |
|
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| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BD Horizon V450 | 406 | 449 |
|
|
| BFP (EBFP) | 380 | 440 |
|
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| BOBO™-1 | 461 | 484 |
|
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY TMR-X | 544 | 573 |
|
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
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| BODIPY TR-X phallacidin | 590 | 621 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
|
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| Calcium Green-1 | 507 | 529 |
|
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| Calcium Orange | 589 | 609 |
|
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| Calcofluor White | 351 | 444 |
|
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| Cascade Blue™ | 401 | 419 |
|
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTrace calcein violet | 400 | 452 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
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| DAF-FM-NO | 495 | 519 |
|
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| DAPI | 359 | 461 |
|
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| Dendra2 (Green) | 491 | 507 |
|
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| Dendra2 (Red) | 554 | 569 |
|
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| DiFMU-pH 9.0 | 358 | 450 |
|
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| DiI | 551 | 569 |
|
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| DiO | 475 | 500 |
|
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| Dronpa | 502 | 516 |
|
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| DsRed | 559 | 583 |
|
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| DsRed-Express | 556 | 584 |
|
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| dTomato | 556 | 582 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
|
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| DyLight 405 | 398 | 420 |
|
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| DyLight 594 | 593 | 618 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
|
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| Ethidium bromide | 518 | 603 |
|
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| Ethidium homodimer | 527 | 617 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| FlAsH-CCPFCC | 511 | 530 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
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| HcRed1 | 588 | 618 |
|
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| KFP-Red | 575 | 599 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
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| LOLO-1 | 568 | 580 |
|
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| LysoSensor Blue | 374 | 424 |
|
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| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Blue | 373 | 422 |
|
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| LysoTracker Green | 501 | 509 |
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| LysoTracker Red | 573 | 592 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Orange | 550 | 574 |
|
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| mApple | 566 | 594 |
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| Marina Blue | 363 | 461 |
|
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| mCherry | 587 | 610 |
|
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| Merocyanine 540 | 559 | 579 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| MitoTracker™ Orange | 551 | 575 |
|
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| MitoTracker™ Red | 578 | 598 |
|
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| mKate2 | 588 | 633 |
|
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| mOrange | 546 | 562 |
|
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| mOrange2 | 551 | 565 |
|
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| mPlum | 589 | 649 |
|
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| mRFP | 555 | 583 |
|
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| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
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| mTFP1 | 462 | 492 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Nile red-triglyceride | 510 | 583 |
|
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| OFP | 547 | 567 |
|
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| Orange 552 | 552 | 574 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| Pacific Blue™ | 404 | 455 |
|
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| pHrodo™, succinimidyl ester | 560 | 587 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| POPO-3 | 533 | 574 |
|
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| Propidium Iodide (PI) | 305 | 617 |
|
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| Pro-Q Diamond | 556 | 583 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| ratiometric pHluorin pH5 | 500 | 509 |
|
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| ReAsH-CCPGCC | 592 | 606 |
|
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| Red 580 | 580 | 603 |
|
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| Resorufin | 571 | 584 |
|
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| Rhod-2 | 553 | 577 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodamine phalloidin | 558 | 575 |
|
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| Rhodamine Red-X | 573 | 591 |
|
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| Rhodol Green | 497 | 524 |
|
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| R-phycoerythrin (PE) | 565 | 576 |
|
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| sgBFP™ | 387 | 451 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| Sirius | 355 | 424 |
|
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
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| Sodium Green | 507 | 532 |
|
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| SpectrumBlue | 405 | 449 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SpectrumOrange | 554 | 587 |
|
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| Sulforhodamine 101-EtOH | 578 | 593 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
|
| SYTOX Orange | 547 | 570 |
|
|
| TagBFP | 402 | 457 |
|
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| Tetramethylrhodamine dextran | 554 | 582 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
|
| TurboFP635(Katushka) | 591 | 638 |
|
|
| TurboGFP | 482 | 503 |
|
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| TurboRFP | 553 | 573 |
|
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| Vybrant DyeCycle Green | 506 | 534 |
|
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| Vybrant DyeCycle Violet | 369 | 437 |
|
|
| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
|
| X-Rhod-1 Indicator | 580 | 601 |
|
|
| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-387/478/555-25
FL-004331 / 0
- Tavg > 80% 382 – 393 nm, Tavg > 90% 466 – 490 nm & Tavg > 90% 546 – 565 nm
- Center Wavelengths of 387.5 nm, 478 nm & 555.5 nm and GMBWs of 11 nm, 24 nm & 19 nm