This 5-filter "Pinkel" set is based on the BrightLine blue-green-orange multiband filter set, but instead of a multiband exciter it has three single-band exciters, a multiband beamsplitter, and a multiband emitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore imaging using a low-noise monochrome camera.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,594.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-433/517/613-25
FL-004334 / 0
- Tavg > 90% 414 – 452 nm, Tavg > 90% 505 – 530 nm & Tavg > 90% 582 – 645 nm
- Center Wavelengths of 433 nm, 517.5 nm & 613.5 nm and GMBWs of 38 nm, 25 nm & 63 nm
FF01-480/17-25
FL-004479 / 0
- Tavg > 92% 471.5 – 488.5 nm
- Center Wavelength of 480 nm and GMBW of 17 nm
- FWHM of 22 nm
FF01-556/20-25
FL-004552 / 0
- Tavg > 93% 546 – 566 nm
- Center Wavelength of 556 nm and GMBW of 20 nm
- FWHM of 25 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF403/497/574-Di01-25x36
FL-007049 / 0
- Dichroic Beamsplitter with edges at 403 nm, 497 nm & 574 nm
- Ravg > 92% 370 – 393 nm, Ravg > 97.5% 466 – 490 nm & Ravg > 97.5% 546 – 565 nm
- Tavg > 90% 414 – 450 nm, Tavg > 90% 507.5 – 528 nm & Tavg > 90% 584 – 645 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
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| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 4-methylumbelliferone | 357 | 441 |
|
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Alexa Fluor® 405 | 401 | 422 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 546 | 556 | 572 |
|
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| Alexa Fluor® 555 | 553 | 565 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 594 | 590 | 618 |
|
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
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| AmCyan1 | 458 | 489 |
|
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 590 | 594 | 624 |
|
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BCECF (pH 9.0) | 502 | 528 |
|
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| BD Horizon V450 | 406 | 449 |
|
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| BFP (EBFP) | 380 | 440 |
|
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY TMR-X | 544 | 573 |
|
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
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| BODIPY TR-X phallacidin | 590 | 621 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
|
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| Calcium Green-1 | 507 | 529 |
|
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| Calcium Orange | 589 | 609 |
|
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| Calcofluor White | 351 | 444 |
|
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| Cascade Blue™ | 401 | 419 |
|
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTrace calcein violet | 400 | 452 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
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| DAF-FM-NO | 495 | 519 |
|
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| DAPI | 359 | 461 |
|
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| Dendra2 (Green) | 491 | 507 |
|
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| Dendra2 (Red) | 554 | 569 |
|
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| DiFMU-pH 9.0 | 358 | 450 |
|
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| DiI | 551 | 569 |
|
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| DiO | 475 | 500 |
|
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| Dronpa | 502 | 516 |
|
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| DsRed | 559 | 583 |
|
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| DsRed-Express | 556 | 584 |
|
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| dTomato | 556 | 582 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
|
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| DyLight 405 | 398 | 420 |
|
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| DyLight 594 | 593 | 618 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
|
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| Ethidium bromide | 518 | 603 |
|
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| Ethidium homodimer | 527 | 617 |
|
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| evoglow-Bs1 | 449 | 496 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| FlAsH-CCPFCC | 511 | 530 |
|
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| Fluo-3 | 506 | 527 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
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| HcRed1 | 588 | 618 |
|
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| HiLyte Fluor™ 594 | 592 | 616 |
|
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| KFP-Red | 575 | 599 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
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| LOLO-1 | 568 | 580 |
|
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| LysoSensor Blue | 374 | 424 |
|
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| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Blue | 373 | 422 |
|
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Red | 573 | 592 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Orange | 550 | 574 |
|
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| mApple | 566 | 594 |
|
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| Marina Blue | 363 | 461 |
|
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| mCherry | 587 | 610 |
|
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| Merocyanine 540 | 559 | 579 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| MitoTracker™ Orange | 551 | 575 |
|
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| MitoTracker™ Red | 578 | 598 |
|
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| mKate2 | 588 | 633 |
|
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| mOrange | 546 | 562 |
|
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| mOrange2 | 551 | 565 |
|
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| mPlum | 589 | 649 |
|
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| mRFP | 555 | 583 |
|
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| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
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| mTFP1 | 462 | 492 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Nile red-triglyceride | 510 | 583 |
|
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| OFP | 547 | 567 |
|
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| Orange 552 | 552 | 574 |
|