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BrightLine® Pinkel filter set, optimized for DAPI, FITC, & TRITC and other like fluorophores

Semrock Part Number: DA/FI/TR-3X-A-000

IDEX Part Number: S-000616  /

Semrock

This 5-filter "Pinkel" set is based on the BrightLine blue-green-orange multiband filter set, but instead of a multiband exciter it has three single-band exciters, a multiband beamsplitter, and a multiband emitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore imaging using a low-noise monochrome camera.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 2,185.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
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Set Components

FF01-387/11-25

FL-004663

Each
  • Tavg > 90% 382 – 393 nm
  • Center Wavelength of 387.5 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FL-004663

FF01-433/517/613-25

FL-004334

Each
  • Tavg > 90% 414 – 452 nm, Tavg > 90% 505 – 530 nm & Tavg > 90% 582 – 645 nm
  • Center Wavelengths of 433 nm, 517.5 nm & 613.5 nm and GMBWs of 38 nm, 25 nm & 63 nm

FL-004334

FF01-480/17-25

FL-004479

Each
  • Tavg > 92% 471.5 – 488.5 nm
  • Center Wavelength of 480 nm and GMBW of 17 nm
  • FWHM of 22 nm

FL-004479

FF01-556/20-25

FL-004552

Each
  • Tavg > 93% 546 – 566 nm
  • Center Wavelength of 556 nm and GMBW of 20 nm
  • FWHM of 25 nm

FL-004552

FF403/497/574-Di01-25x36

FL-007049

Each
  • Dichroic Beamsplitter with edges at 403 nm, 497 nm & 574 nm
  • Ravg > 92% 370 – 393 nm, Ravg > 97.5% 466 – 490 nm & Ravg > 97.5% 546 – 565 nm
  • Tavg > 90% 414 – 450 nm, Tavg > 90% 507.5 – 528 nm & Tavg > 90% 584 – 645 nm

FL-007049

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

DA/FI/TR-3X-A-NQF

S-001340 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

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DA/FI/TR-3X-A-NTE

S-000145 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

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DA/FI/TR-3X-A-OMF

S-000147 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

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DA/FI/TR-3X-A-ZHE

S-001342 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

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DA/FI/TR-3X-A-OFF

S-000146 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 488 500 525
ATTO 495 495 527
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-3 570 605
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Calcium Orange 589 609
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace BODIPY TR methyl ester 598 625
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
DiFMU-pH 9.0 358 450
DiI 551 569
DiO 475 500
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-505-Phalloidin 503 530
DY-590 582 599
DyLight 405 398 420
DyLight 594 593 618
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 405 403 428
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
KFP-Red 575 599
LIVE-DEAD Fixable Green Dead Cell Stain 498 525 </