This 7-filter "Sedat" set is based on the BrightLine blue-green-orange multiband filter set, but instead of multiband exciter and emitter filters, it has three single-band exciters, three single-band emitters, and a triple-band beamsplitter. With its high
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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3,079.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-435/40-25
FL-003733 / 0
- Tavg > 90% 415 – 455 nm
- Center Wavelength of 435 nm and GMBW of 40 nm
- FWHM of 48.4 nm
FF02-520/28-25
FL-004295 / 0
- Tavg > 93% 506 – 534 nm
- Center Wavelength of 520 nm and GMBW of 28 nm
- FWHM of 34.7 nm
FF02-617/73-25
FL-004462 / 0
- Tavg > 90% 580.5 – 653.5 nm
- Center Wavelength of 617 nm and GMBW of 73 nm
- FWHM of 79 nm
FF01-480/17-25
FL-004479 / 0
- Tavg > 92% 471.5 – 488.5 nm
- Center Wavelength of 480 nm and GMBW of 17 nm
- FWHM of 22 nm
FF01-556/20-25
FL-004552 / 0
- Tavg > 93% 546 – 566 nm
- Center Wavelength of 556 nm and GMBW of 20 nm
- FWHM of 25 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 381.5 – 392.5 nm
- Center Wavelength of 387 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF403/497/574-Di01-25x36
FL-007049 / 0
- Dichroic Beamsplitter with edges at 403 nm, 497 nm & 574 nm
- Ravg > 92% 370 – 393 nm, Ravg > 97.5% 466 – 490 nm & Ravg > 97.5% 546 – 565 nm
- Tavg > 90% 414 – 450 nm, Tavg > 90% 507.5 – 528 nm & Tavg > 90% 584 – 645 nm
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 2-dodecylresorufin-lipid | 582 | 595 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 546 | 556 | 572 |
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| Alexa Fluor® 555 | 553 | 565 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| AmCyan1 | 458 | 489 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| AsRed 2 | 576 | 592 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 550 | 553 | 576 |
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| ATTO 565 | 563 | 592 |
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| ATTO 590 | 594 | 624 |
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| BCECF (pH 5.5) | 481 | 518 |
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| BCECF (pH 9.0) | 502 | 528 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| BODIPY TMR-X | 544 | 573 |
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| BODIPY TR-X (MeOH) | 544 | 573 |
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| BODIPY TR-X phallacidin | 590 | 621 |
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| Calcein | 494 | 514 |
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| Calcium Crimson | 589 | 609 |
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| Calcium Green-1 | 507 | 529 |
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| Calcium Orange | 589 | 609 |
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| Calcofluor White | 351 | 444 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
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| CellTrace calcein violet | 400 | 452 |
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