BrightLine® Sedat filter set, optimized for DAPI, FITC, & TRITC and other like fluorophores

Semrock Part Number: DA/FI/TR-3X3M-C-NQF

IDEX Part Number: S-001346  /

Semrock

This 7-filter "Sedat" set is based on the BrightLine blue-green-orange multiband filter set, but instead of multiband exciter and emitter filters, it has three single-band exciters, three single-band emitters, and a triple-band beamsplitter. With its high

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FF02-435/40-25

FL-003733 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 415 – 455 nm
  • Center Wavelength of 435 nm and GMBW of 40 nm
  • FWHM of 48.4 nm

FF02-520/28-25

FL-004295 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 506 – 534 nm
  • Center Wavelength of 520 nm and GMBW of 28 nm
  • FWHM of 34.7 nm

FF02-617/73-25

FL-004462 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 580.5 – 653.5 nm
  • Center Wavelength of 617 nm and GMBW of 73 nm
  • FWHM of 79 nm

FF01-480/17-25

FL-004479 / 0

Out of Stock Backordered In Stock
  • Tavg > 92% 471.5 – 488.5 nm
  • Center Wavelength of 480 nm and GMBW of 17 nm
  • FWHM of 22 nm

FF01-556/20-25

FL-004552 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 546 – 566 nm
  • Center Wavelength of 556 nm and GMBW of 20 nm
  • FWHM of 25 nm

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF403/497/574-Di01-25x36

FL-007049 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edges at 403 nm, 497 nm & 574 nm
  • Ravg > 92% 370 – 393 nm, Ravg > 97.5% 466 – 490 nm & Ravg > 97.5% 546 – 565 nm
  • Tavg > 90% 414 – 450 nm, Tavg > 90% 507.5 – 528 nm & Tavg > 90% 584 – 645 nm

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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm

1.1 mm
(Can accept a 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592