This "full-multiband" filter set allows for simultaneous direct viewing or imaging of DAPI and FITC with a color camera.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,874.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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Clear selection
Select all 3 products from all pages.
FF01-387/480-25
FL-004187 / 0
- Tavg > 80% 381.5 – 392.5 nm & Tavg > 90% 466 – 495 nm
- Center Wavelengths of 387 nm & 480 nm and GMBWs of 11 nm & 29 nm
FF01-433/530-25
FL-004229 / 0
- Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm
- Center Wavelengths of 433 nm & 530 nm and GMBWs of 38 nm & 40 nm
FF403/502-Di01-25x36
FL-006945 / 0
- Dichroic Beamsplitter with edges at 403 nm & 502 nm
- Ravg > 97.5% 370 – 393 nm & Ravg > 97.5% 466 – 495 nm
- Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm
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This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| AmCyan1 | 458 | 489 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| BCECF (pH 5.5) | 481 | 518 |
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| BCECF (pH 9.0) | 502 | 528 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| BOBO™-1 | 461 | 484 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| Calcein | 494 | 514 |
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| Calcium Green-1 | 507 | 529 |
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| Calcofluor White | 351 | 444 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
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| CFP2 | 490 | 510 |
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| Cy2™ | 492 | 507 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| DAPI | 359 | 461 |
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| Dendra2 (Green) | 491 | 507 |
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| DiA | 457 | 586 |
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| DiFMU-pH 9.0 | 358 | 450 |
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
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| DyLight 405 | 398 | 420 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
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| FITC (Fluorescein) | 495 | 519 |
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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| FM 1-43 | 473 | 579 |
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| GFP (EGFP) | 489 | 511 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HiLyte Fluor™ 405 | 403 | 428 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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| LysoSensor Blue | 374 | 424 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Blue | 373 | 422 |
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| LysoTracker Green | 501 | 509 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Green | 507 | 531 |
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| Marina Blue | 363 | 461 |
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Green | 490 | 512 |
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| mTFP1 | 462 | 492 |
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Oregon Green™ 488 | 498 | 526 |
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| Pacific Blue™ | 404 | 455 |
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| PicoGreen® | 502 | 522 |
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| PKH67 | 488 | 500 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
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| ratiometric pHluorin pH5 | 500 | 509 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine 123 | 507 | 529 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| sgBFP™ | 387 | 451 |
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| sgGFP™ (super glow GFP) | 472 | 506 |
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| Sirius | 355 | 424 |
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| Sodium Green | 507 | 532 |
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| SpectrumBlue | 405 | 449 |
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| SpectrumGreen | 497 | 538 |
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
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| SYTO 11 | 506 | 525 |
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| SYTO 13 | 448 | 506 |
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| SYTO 16 | 489 | 520 |
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| SYTO 9 | 483 | 500 |
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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| SYTOX Green-DNA | 504 | 524 |
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| TagBFP | 402 | 457 |
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| TO-PRO-1 | 515 | 531 |
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| TOTO-1 | 514 | 531 |
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| TurboGFP | 482 | 503 |
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| Vybrant DyeCycle Green | 506 | 534 |
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| Vybrant DyeCycle Violet | 369 | 437 |
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
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| YO-PRO-1 | 491 | 506 |
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| YOYO-1 | 491 | 508 |
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total selected
Clear selection
Select all 3 products from all pages.
FF01-387/480-25
FL-004187 / 0
- Tavg > 80% 381.5 – 392.5 nm & Tavg > 90% 466 – 495 nm
- Center Wavelengths of 387 nm & 480 nm and GMBWs of 11 nm & 29 nm
FF01-433/530-25
FL-004229 / 0
- Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm
- Center Wavelengths of 433 nm & 530 nm and GMBWs of 38 nm & 40 nm
FF403/502-Di01-25x36
FL-006945 / 0
- Dichroic Beamsplitter with edges at 403 nm & 502 nm
- Ravg > 97.5% 370 – 393 nm & Ravg > 97.5% 466 – 495 nm
- Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm