This 4-filter "Pinkel" set is perfectly optimized for imaging DAPI and FITC with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is comprised of two single-band exciters along with a dual-band beamsplitter and a dual-band emitter, and thus works best with an exciter filter wheel or slider.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,084.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-433/530-25
FL-004229 / 0
- Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm
- Center Wavelengths of 433 nm & 530 nm and GMBWs of 38 nm & 40 nm
FF02-485/20-25
FL-004432 / 0
- Tavg > 93% 475 – 495 nm
- Center Wavelength of 485 nm and GMBW of 20 nm
- FWHM of 24.9 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF403/502-Di01-25x36
FL-006945 / 0
- Dichroic Beamsplitter with edges at 403 nm & 502 nm
- Ravg > 97.5% 370 – 393 nm & Ravg > 97.5% 466 – 495 nm
- Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 514 | 518 | 543 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| AmCyan1 | 458 | 489 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| BCECF (pH 5.5) | 481 | 518 |
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| BCECF (pH 9.0) | 502 | 528 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| Calcein | 494 | 514 |
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| Calcium Green-1 | 507 | 529 |
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| Calcofluor White | 351 | 444 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
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| CFP2 | 490 | 510 |
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| Cy2™ | 492 | 507 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| DAPI | 359 | 461 |
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| Dendra2 (Green) | 491 | 507 |
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| DiFMU-pH 9.0 | 358 | 450 |
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
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| DyLight 405 | 398 | 420 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
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| FITC (Fluorescein) | 495 | 519 |
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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| FM 1-43 | 473 | 579 |
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| GFP (EGFP) | 489 | 511 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HiLyte Fluor™ 405 | 403 | 428 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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| LysoSensor Blue | 374 | 424 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Blue | 373 | 422 |
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| LysoTracker Green | 501 | 509 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Green | 507 | 531 |
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| Marina Blue | 363 | 461 |
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Green | 490 | 512 |
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Oregon Green™ 488 | 498 | 526 |
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| Oregon Green™ 514 | 513 | 532 |
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| Pacific Blue™ | 404 | 455 |
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| PicoGreen® | 502 | 522 |
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| PKH67 | 488 | 500 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| ratiometric pHluorin pH5 | 500 | 509 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine 123 | 507 | 529 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| sgBFP™ | 387 | 451 |
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| sgGFP™ (super glow GFP) | 472 | 506 |
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| Sirius | 355 | 424 |
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| Sodium Green | 507 | 532 |
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| SpectrumBlue | 405 | 449 |
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| SpectrumGreen | 497 | 538 |
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
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| SYTO 11 | 506 | 525 |
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| SYTO 13 | 448 | 506 |
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| SYTO 16 | 489 | 520 |
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| SYTO 9 | 483 | 500 |
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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| SYTOX Green-DNA | 504 | 524 |
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| TagBFP | 402 | 457 |
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| TO-PRO-1 | 515 | 531 |
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| TOTO-1 | 514 | 531 |
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| TurboGFP | 482 | 503 |
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| Vybrant DyeCycle Green | 506 | 534 |
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| Vybrant DyeCycle Violet | 369 | 437 |
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| YFP (yellow GFP) | 513 | 530 |
|
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| YO-PRO-1 | 491 | 506 |
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| YOYO-1 | 491 | 508 |
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total selected
Clear selection
Select all 4 products from all pages.
FF01-433/530-25
FL-004229 / 0
- Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm
- Center Wavelengths of 433 nm & 530 nm and GMBWs of 38 nm & 40 nm
FF02-485/20-25
FL-004432 / 0
- Tavg > 93% 475 – 495 nm
- Center Wavelength of 485 nm and GMBW of 20 nm
- FWHM of 24.9 nm