This 5-filter "Sedat" set is ideal for high-speed sequential imaging DAPI and FITC with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is comprised of two single-band exciters and two single-band emitters, along with a dual
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 5.0 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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1,8-ANS | 372 | 477 |
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4-methylumbelliferone | 357 | 441 |
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5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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6-carboxyrhodamine 6G | 525 | 555 |
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7-hydroxy-4-methylcoumarin | 360 | 442 |
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Acridine Orange (+DNA) | 502 | 526 |
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Alexa Fluor® 405 | 401 | 422 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 514 | 518 | 543 |
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AMCA (Aminomethylcoumarin) | 350 | 448 |
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AmCyan1 | 458 | 489 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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BCECF (pH 5.5) | 481 | 518 |
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BCECF (pH 9.0) | 502 | 528 |
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BD Horizon V450 | 406 | 449 |
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BFP (EBFP) | 380 | 440 |
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BODIPY FL | 505 | 512 |
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BODIPY FL-MeOH | 502 | 511 |
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Calcein | 494 | 514 |
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Calcium Green-1 | 507 | 529 |
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Calcofluor White | 351 | 444 |
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Cascade Blue™ | 401 | 419 |
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CellTrace calcein violet | 400 | 452 |
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CFP2 | 490 | 510 |
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Cy2™ | 492 | 507 |
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CyQUANT GR-DNA | 502 | 523 |
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DAF-FM-NO | 495 | 519 |
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DAPI | 359 | 461 |
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Dendra2 (Green) | 491 | 507 |
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DiFMU-pH 9.0 | 358 | 450 |
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DiO | 475 | 500 |
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Dronpa | 502 | 516 |
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DY-505-Phalloidin | 503 | 530 |
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DyLight 405 | 398 | 420 |
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ecliptic pHluorin pH5.5 | 473 | 507 |
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Emerald | 491 | 511 |
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FITC (Fluorescein) | 495 | 519 |
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FlAsH-CCPFCC | 511 | 530 |
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Fluo-3 | 506 | 527 |
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Fluo-4 | 494 | 516 |
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Fluorescein dextran | 501 | 524 |
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Fluorescein-pH 8.0 | 489 | 517 |
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Fluoro-Emerald | 494 | 518 |
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FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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FM 1-43 | 473 | 579 |
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GFP (EGFP) | 489 | 511 |
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Green 496 | 496 | 520 |
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Green 500 | 501 | 524 |
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HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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HCS LipidTOX Green phospholipidosis | 504 | 536 |
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HiLyte Fluor™ 405 | 403 | 428 |
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HiLyte Fluor™ 488 | 497 | 526 |
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LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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LysoSensor Blue | 374 | 424 |
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LysoSensor Green | 448 | 502 |
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LysoTracker Blue | 373 | 422 |
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LysoTracker Green | 501 | 509 |
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LysoTracker Yellow HCK-123 | 488 | 565 |
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Magnesium Green | 507 | 531 |
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Marina Blue | 363 | 461 |
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mHoneyDew | 478 | 562 |
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MitoTracker™ Green | 490 | 512 |
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mWasabi | 493 | 509 |
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NBD-X (MeOH) | 467 | 538 |
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NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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Oregon Green™ 488 | 498 | 526 |
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Oregon Green™ 514 | 513 | 532 |
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Pacific Blue™ | 404 | 455 |
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PicoGreen® | 502 | 522 |
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PKH67 | 488 | 500 |
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Qdot® 525 Nanocrystals | 300 | 525 |
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ratiometric pHluorin pH5 | 500 | 509 |
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Rhodamine 110 | 497 | 519 |
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Rhodamine 123 | 507 | 529 |
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Rhodamine Green | 497 | 523 |
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Rhodol Green | 497 | 524 |
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sgBFP™ | 387 | 451 |
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sgGFP™ (super glow GFP) | 472 | 506 |
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Sirius | 355 | 424 |
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Sodium Green | 507 | 532 |
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SpectrumBlue | 405 | 449 |
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SpectrumGreen | 497 | 538 |
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SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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SYBR Safe DNA gel stain-DNA | 509 | 526 |
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SYTO 11 | 506 | 525 |
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SYTO 13 | 448 | 506 |
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SYTO 16 | 489 | 520 |
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SYTO 9 | 483 | 500 |
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SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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SYTOX Green-DNA | 504 | 524 |
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TagBFP | 402 | 457 |
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TO-PRO-1 | 515 | 531 |
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TOTO-1 | 514 | 531 |
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TurboGFP | 482 | 503 |
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Vybrant DyeCycle Green | 506 | 534 |
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Vybrant DyeCycle Violet | 369 | 437 |
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wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
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YFP (yellow GFP) | 513 | 530 |
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YO-PRO-1 | 491 | 506 |
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YOYO-1 | 491 | 508 |
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FL-003733 / 0