This filter set is ideal for use with Cy7™ and Alexa Fluor 750, as well as other similar near-infrared fluorophores. The longer excitation and emission wavelengths of these fluorophores enable deeper tissue penetration. The superb brightness and excellent signal-to-noise ratio of this filter set is also ideal since deep tissue imaging applications often also tend to yield very low light levels.
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This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||25.0 mm||5.0 mm|
|Emitter (Diameter)||25.0 mm||5.0 mm|
|Dichroic (L × W)||25.2 mm × 35.6 mm||1.5 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|
|What is Pixel Shift, and how does the Semrock ZERO™ option help?||Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.|