BrightLine Basic^™ single-band filter set, optimized for Cy5 and other like fluorophores

Semrock Part Number: Cy5-B-Basic-000

IDEX Part Number: S-443172 /

FFB01-627/36-25 FL-442463 Each T_avg > 93% 609 – 645 nm Center Wavelength of 627 nm and GMBW of 36 nm FWHM of 42.3 nm	FL-442463	1 Each
FFB01-699/54-25 FL-442464 Each T_avg > 93% 672 – 726 nm Center Wavelength of 699 nm and GMBW of 54 nm FWHM of 61 nm	FL-442464	1 Each
FF658-Di01-25x36 FL-442465 Each Dichroic Beamsplitter with edge at 658 nm R_avg > 98% 350 – 648 nm T_avg > 95% 667 – 1100 nm Flatness / RWE: Standard Epi-fluorescence	FL-442465	1 Each

Optical Specifications	Value
Blocking	BrightLine® Basic filters offer essential performance specifications. These sets are suitable for various fluorescence applications ranging from detection to imaging.
General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications	Value
Scratch-Dig	80-50
Dichroic Orientation	Reflective coating side should face toward light source.

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density	Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO^™ option help?	Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
5-carboxynapthofluorescein (pH 10)	555	615
Alexa Fluor^® 647	653	668
Alexa Fluor^® 660	663	691
Alexa Fluor^® 680	679	702
APC (Allophycocyanin)	651	660
ATTO 620	618	642
ATTO 633	629	658
ATTO 635	635	658
ATTO 647	644	670
ATTO 647N	645	668
ATTO 655	663	683
ATTO 680	680	700
BODIPY 650/665-X	646	664
Carboxynaphthofluorescein	599	674
Cy5.5™	672	690
Cy5™	649	666
DiD (DiIC18(5)) - Lipophilic Tracer	648	670
DyLight 649	651	673
DyLight 680	676	705
FluoSpheres Crimson fluorescent microspheres	620	646
FluoSpheres Dark Red fluorescent microspheres	656	683
HCS CellMask Red cytoplasmic	622	644
HiLyte Fluor™ 647	649	673
LDS 751	560	711
LIVE-DEAD Fixable Far Red Dead Cell Stain	653	668
MitoTracker™ Deep Red	641	662
Nile blue-EtOH	631	660
Qdot^® 705 Nanocrystals	300	702
Red 650	649	665
SpectrumFRed (Far Red)	650	673
SYTO 59	622	644
SYTO 60	650	681
SYTO 61	618	650
SYTOX Red-DNA	640	658
TO-PRO-3	642	657
TOTO-3	642	661

Optical Specifications: Value
Blocking: BrightLine® Basic filters offer essential performance specifications. These sets are suitable for various fluorescence applications ranging from detection to imaging.
General Filter Specifications: Value
Optical Damage Rating: Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility: BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications: Value
Scratch-Dig: 80-50
Dichroic Orientation: Reflective coating side should face toward light source.

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density	Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO^™ option help?	Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

FFB01-627/36-25