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BrightLine Basic™ single-band filter set, optimized for Cy5 and other like fluorophores

Semrock Part Number: Cy5-B-Basic-000

IDEX Part Number: S-443172  /

Semrock

Our Brightline® Basic fluorescence filter set belongs to our essential performance group. These sets are suitable for various fluorescence applications ranging from detection to imaging. These filters feature a fundamental and basic design, offered at a competitive price point. They are designed with thin-film hard-coated technology, they ensure exceptional performance and reliability.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 553.18 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FFB01-627/36-25

FL-442463

Each
  • Tavg > 93% 609 – 645 nm
  • Center Wavelength of 627 nm and GMBW of 36 nm
  • FWHM of 44.9 nm

FL-442463

FFB01-699/54-25

FL-442464

Each
  • Tavg > 93% 672 – 726 nm
  • Center Wavelength of 699 nm and GMBW of 54 nm
  • FWHM of 63.8 nm

FL-442464

FF658-Di01-25x36

FL-442465

Each
  • Dichroic Beamsplitter with edge at 658 nm
  • Ravg > 98% 350 – 648 nm
  • Tavg > 95% 667 – 1100 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-442465

Optical Specifications Value
Blocking BrightLine Basic™ filters offer essential performance specifications. These sets are suitable for various fluorescence applications ranging from detection to imaging.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 80-50
Dichroic Orientation Reflective coating side should face toward light source.
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxynapthofluorescein (pH 10) 555 615
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
APC (Allophycocyanin) 651 660
ATTO 620 618 642
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
BODIPY 650/665-X 646 664
Carboxynaphthofluorescein 599 674
Cy5.5™ 672 690
Cy5™ 649 666
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DyLight 649 651 673
DyLight 680 676 705
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Dark Red fluorescent microspheres 656 683
HCS CellMask Red cytoplasmic 622 644
HiLyte Fluor™ 647 649 673
LDS 751 560 711
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
MitoTracker™ Deep Red 641 662
Nile blue-EtOH 631 660
Qdot® 705 Nanocrystals 300 702
Red 650 649 665
SpectrumFRed (Far Red) 650 673
SYTO 59 622 644
SYTO 60 650 681
SYTO 61 618 650
SYTOX Red-DNA 640 658
TO-PRO-3 642 657
TOTO-3 642 661

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

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