BrightLine® single-band filter set, optimized for Cy5.5 and other like fluorophores

Semrock Part Number: Cy5.5-C-OFF-ZERO

IDEX Part Number: S-000096  /

Semrock

This filter set is ideal for use with Cy5.5™ and Alexa Fluor™ 680, as well as other deep-red/near-infrared fluorophores. The longer excitation and emission wavelengths of these fluorophores enable deeper tissue penetration. The superb brightne

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FF02-655/40-25

FL-004366 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 635 – 675 nm
  • Center Wavelength of 655 nm and GMBW of 40 nm
  • FWHM of 46.9 nm

FF01-716/40-25

FL-004457 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 696 – 736 nm
  • Center Wavelength of 716 nm and GMBW of 40 nm
  • FWHM of 47.2 nm

FF685-Di02-25x36

FL-007099 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 685 nm
  • Ravg > 98% 350 – 676 nm
  • Tavg > 93% 695 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
Alexa Fluor® 700 696 719
ATTO 635 635 658
ATTO 647 644 670
ATTO 655 663 683
ATTO 680 680 700
ATTO 700 699 719
Cy5.5™ 672 690
DY-675 675 699
DyLight 680 676 705
FluoSpheres Dark Red fluorescent microspheres 656 683
HiLyte Fluor™ 680 688 702
IRDye 680 NHS Ester 683 710
IRDye 700Phosphoramidite 691 699
Qdot® 705 Nanocrystals 300 702
Quasar® 705 688 706
SYTO 60 650 681

Related Products

FF02-655/40-25

FL-004366 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 635 – 675 nm
  • Center Wavelength of 655 nm and GMBW of 40 nm
  • FWHM of 46.9 nm

FF01-716/40-25

FL-004457 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 696 – 736 nm
  • Center Wavelength of 716 nm and GMBW of 40 nm
  • FWHM of 47.2 nm

FF685-Di02-25x36

FL-007099 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 685 nm
  • Ravg > 98% 350 – 676 nm
  • Tavg > 93% 695 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.