BrightLine® single-band filter set, optimized for Cy5.5 and other like fluorophores

Semrock Part Number: Cy5.5-C-NQF

IDEX Part Number: S-001295  /

Semrock
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
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  • Price Not Available.
  • 1,464.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)
1.1 mm
(Can accept a 2mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
Alexa Fluor® 700 696 719
ATTO 635 635 658
ATTO 647 644 670
ATTO 655 663 683
ATTO 680 680 700
ATTO 700 699 719
Cy5.5™ 672 690
DY-675 675 699
DyLight 680 676 705
FluoSpheres Dark Red fluorescent microspheres 656 683
HiLyte Fluor™ 680 688 702
IRDye 680 NHS Ester 683 710
IRDye 700Phosphoramidite 691 699
Qdot® 705 Nanocrystals 300 702
Quasar® 705 688 706
SYTO 60 650 681

NQF Holder/Cube

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)
1.1 mm
(Can accept a 2mm dichroic)