BrightLine^® 2.0 single-band filter set, optimized for Cy5 and other like fluorophores

Semrock Part Number: Cy5-4040D-000

IDEX Part Number: S-443178 /

FF03-628/40-25 FL-442467 Each T_avg > 95% 608 – 648 nm Center Wavelength of 628 nm and GMBW of 40 nm FWHM of 46.3 nm	FL-442467	1 Each
FF01-688/41-25 FL-442466 Each T_avg > 95% 667 – 708 nm Center Wavelength of 687.5 nm and GMBW of 41 nm FWHM of 47.9 nm	FL-442466	1 Each
FF658-Di01-25x36 FL-442465 Each Dichroic Beamsplitter with edge at 658 nm R_avg > 98% 350 – 648 nm T_avg > 95% 667 – 1100 nm Flatness / RWE: Standard Epi-fluorescence	FL-442465	1 Each

Optical Specifications	Value
Blocking	BrightLine® 2.0 filters have blocking far exceeding OD 8 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility	BrightLine® 2.0 filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications	Value
Scratch-Dig	60-40
Dichroic Orientation	Reflective coating side should face toward light source.

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density	Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO^™ option help?	Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
5-carboxynapthofluorescein (pH 10)	555	615
Alexa Fluor^® 647	653	668
Alexa Fluor^® 660	663	691
Alexa Fluor^® 680	679	702
APC	651	660
APC (Allophycocyanin)	651	660
ATTO 620	618	642
ATTO 633	629	658
ATTO 635	635	658
ATTO 647	644	670
ATTO 647N	645	668
ATTO 655	663	683
ATTO 680	680	700
BODIPY 650/665-X	646	664
Carboxynaphthofluorescein	599	674
Cy5.5™	672	690
Cy5™	649	666
DDAO	648	657
DiD (DiIC18(5)) - Lipophilic Tracer	648	670
DY-630	634	656
DyLight 649	651	673
DyLight 680	676	705
FluoSpheres Crimson fluorescent microspheres	620	646
FluoSpheres Dark Red fluorescent microspheres	656	683
HCS CellMask Red cytoplasmic	622	644
HiLyte Fluor™ 647	649	673
LDS 751	560	711
LIVE-DEAD Fixable Far Red Dead Cell Stain	653	668
MitoTracker™ Deep Red	641	662
Nile blue-EtOH	631	660
Qdot^® 705 Nanocrystals	300	702
Red 650	649	665
SpectrumFRed (Far Red)	650	673
SYTO 59	622	644
SYTO 60	650	681
SYTO 61	618	650
SYTO Red Fluorescent Nucleic Acid Stains 60	655	675
SYTO Red Fluorescent Nucleic Acid Stains 62	655	675
SYTO Red Fluorescent Nucleic Acid Stains 63	655	675
SYTOX Red-DNA	640	658
TO-PRO-3	642	657
TOTO-3	642	661

Optical Specifications: Value
Blocking: BrightLine® 2.0 filters have blocking far exceeding OD 8 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications: Value
Optical Damage Rating: Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility: BrightLine® 2.0 filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications: Value
Scratch-Dig: 60-40
Dichroic Orientation: Reflective coating side should face toward light source.

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density	Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO^™ option help?	Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

FF03-628/40-25