{{product.AlternativeText}}
{{product.AlternativeText}}

/ 

|
OR Continue Checkout View Cart ()

All {{categoryNode2.CategoryNodeInfo.Description}}

All {{categoryNode2.CategoryNodeInfo.Description}}
{{productSuggestion.AlternativeText}}
{{productSuggestion.AlternativeText}}

/ 

  Switch Customer

  | 

Go to recently viewed Go to wish list Go to product list
Switch Customer
{{product.AlternativeText}}
{{product.AlternativeText}}

/ 

|
OR Continue Checkout View Cart ()

All {{categoryNode2.CategoryNodeInfo.Description}}

BrightLine® 2.0 single-band filter set, optimized for Cy5 and other like fluorophores

Semrock Part Number: Cy5-4040D-000

IDEX Part Number: S-443178  /

Semrock

BrightLine™ 2.0 filter sets introduces a unified, improved design that delivers enhanced contrast and leverages the latest advances in coating technologies across this industry-leading filter family. The fluorescence bandpass filters and sets are meticulously optimized for seamless integration across a wide range of fluorescence instruments. With its all-thin-film, hard-coated construction, BrightLine 2.0 sets a new standard in performance and reliability.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 908.43 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • 0.00
    0.00
  • Notify me when available
We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Add to List Add to List
Set Components

FF03-628/40-25

FL-442467

Each
  • Tavg > 95% 608 – 648 nm
  • Center Wavelength of 628 nm and GMBW of 40 nm
  • FWHM of 46.6 nm

FL-442467

FF01-688/41-25

FL-442466

Each
  • Tavg > 95% 667 – 708 nm
  • Center Wavelength of 687.5 nm and GMBW of 41 nm
  • FWHM of 48 nm

FL-442466

FF658-Di01-25x36

FL-442465

Each
  • Dichroic Beamsplitter with edge at 658 nm
  • Ravg > 98% 350 – 648 nm
  • Tavg > 95% 667 – 1100 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-442465

Optical Specifications Value
Blocking BrightLine® 2.0 filters have blocking far exceeding OD 8 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine® 2.0 filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxynapthofluorescein (pH 10) 555 615
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
APC 651 660
APC (Allophycocyanin) 651 660
ATTO 620 618 642
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
BODIPY 650/665-X 646 664
Carboxynaphthofluorescein 599 674
Cy5.5™ 672 690
Cy5™ 649 666
DDAO 648 657
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DY-630 634 656
DyLight 649 651 673
DyLight 680 676 705
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Dark Red fluorescent microspheres 656 683
HCS CellMask Red cytoplasmic 622 644
HiLyte Fluor™ 647 649 673
LDS 751 560 711
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
MitoTracker™ Deep Red 641 662
Nile blue-EtOH 631 660
Qdot® 705 Nanocrystals 300 702
Red 650 649 665
SpectrumFRed (Far Red) 650 673
SYTO 59 622 644
SYTO 60 650 681
SYTO 61 618 650
SYTO Red Fluorescent Nucleic Acid Stains 60 655 675
SYTO Red Fluorescent Nucleic Acid Stains 62 655 675
SYTO Red Fluorescent Nucleic Acid Stains 63 655 675
SYTOX Red-DNA 640 658
TO-PRO-3 642 657
TOTO-3 642 661

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

You might also be interested in the following products

{{crossRefProduct.AlternativeText}} {{crossRefProduct.HoverImageAlternativeText}}

/

Recently Viewed

View All

PFA HP+ Tubing 360µm OD x 100µm ID

1932