BrightLine® single-band filter set, optimized for Cy5 and other like fluorophores

Semrock Part Number: Cy5-4040C-OFF-ZERO

IDEX Part Number: S-000105  /

Semrock

This filter set is ideal for use with Cy5™ and other deep red fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.

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  • 1,623.00 USD Each
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FF02-628/40-25

FL-004435 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 608 – 648 nm
  • Center Wavelength of 628 nm and GMBW of 40 nm
  • FWHM of 46.5 nm

FF01-692/40-25

FL-004702 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 672 – 712 nm
  • Center Wavelength of 692 nm and GMBW of 40 nm
  • FWHM of 47.6 nm

FF660-Di02-25x36

FL-007041 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 660 nm
  • Ravg > 98% 350 – 651 nm
  • Tavg > 93% 669 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)26 mm x 38 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxynapthofluorescein (pH 10) 555 615
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
APC 651 660
APC (Allophycocyanin) 651 660
ATTO 620 618 642
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
BODIPY 650/665-X 646 664
Carboxynaphthofluorescein 599 674
Cy5.5â„¢ 672 690
Cy5â„¢ 649 666
DDAO 648 657
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DY-630 634 656
DyLight 649 651 673
DyLight 680 676 705
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Dark Red fluorescent microspheres 656 683
HCS CellMask Red cytoplasmic 622 644
HiLyte Fluorâ„¢ 647 649 673
LDS 751 560 711
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
MitoTrackerâ„¢ Deep Red 641 662
Nile blue-EtOH 631 660
Qdot® 705 Nanocrystals 300 702
Red 650 649 665
SpectrumFRed (Far Red) 650 673
SYTO 59 622 644
SYTO 60 650 681
SYTO 61 618 650
SYTO Red Fluorescent Nucleic Acid Stains 60 655 675
SYTO Red Fluorescent Nucleic Acid Stains 62 655 675
SYTO Red Fluorescent Nucleic Acid Stains 63 655 675
SYTOX Red-DNA 640 658
TO-PRO-3 642 657
TOTO-3 642 661

Related Products

FF02-628/40-25

FL-004435 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 608 – 648 nm
  • Center Wavelength of 628 nm and GMBW of 40 nm
  • FWHM of 46.5 nm

FF01-692/40-25

FL-004702 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 672 – 712 nm
  • Center Wavelength of 692 nm and GMBW of 40 nm
  • FWHM of 47.6 nm

FF660-Di02-25x36

FL-007041 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 660 nm
  • Ravg > 98% 350 – 651 nm
  • Tavg > 93% 669 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)26 mm x 38 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.