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BrightLine® full-multiband filter set, optimized for Cy3 & Cy5 and other like fluorophores

Semrock Part Number: Cy3/Cy5-A-000

IDEX Part Number: S-000687  /

Semrock

This new "full-multiband" filter set allows for simultaneous direct viewing or imaging with a color camera.

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Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxynapthofluorescein (pH 10) 555 615
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 633 631 647
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660
ATTO 520 516 538
ATTO 532 501 545
ATTO 550 553 576
ATTO 565 563 592
ATTO 620 618 642
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
BODIPY 650/665-X 646 664
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
Calcium Orange 589 609
Carboxynaphthofluorescein 599 674
Cy3.5™ 578 591
Cy3™ 554 566
Cy5.5™ 672 690
Cy5™ 649 666
DDAO 648 657
Dendra2 (Red) 554 569
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DiI 551 569
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-530 537 560
DY-630 634 656
DY-675 675 699
DyLight 549 553 569
DyLight 649 651 673
DyLight 680 676 705
Eosin 525 546
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Dark Red fluorescent microspheres 656 683
FluoSpheres Orange fluorescent microspheres 540 559
Gold 525 521 551
Gold 550 551 564
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HiLyte Fluor™ 555 550 566
HiLyte Fluor™ 647 649 673
HiLyte Fluor™ 680 688 702
IRDye 680 NHS Ester 683 710
IRDye 700Phosphoramidite 691 699
KFP-Red 575 599
LDS 751 560 711
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
LOLO-1 568 580
Magnesium Orange 550 574
mApple 566 594
Merocyanine 540 559 579
mHoneyDew 478 562
MitoTracker™ Deep Red 641 662
MitoTracker™ Orange 551 575
mKO 549 559
mOrange 546 562
mOrange2 551 565
mRFP 555 583
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile blue-EtOH 631 660
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
pHrodo™, succinimidyl ester 560 587
POPO-3 533 574
PO-PRO-3 539 570
Pro-Q Diamond 556 583
Qdot® 565 Nanocrystals 300 564
Qdot® 585 Nanocrystals 300 588
Qdot® 705 Nanocrystals 300 702
Red 650 649 665
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
R-phycoerythrin (PE) 565 576
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 514nm (ph 6.0) 549 587
SpectrumFRed (Far Red) 650 673
SpectrumGold 530 556
SpectrumOrange 554 587
SYTO 59 622 644
SYTO 60 650 681
SYTO 61 618 650
SYTO 82 540 559
SYTOX Orange 547 570
SYTOX Red-DNA 640 658
Tetramethylrhodamine dextran 554 582
TO-PRO-1 515 531
TO-PRO-3 642 657
TOTO-1 514 531
TOTO-3 642 661
TRITC (Tetramethylrhodamine) 552 578
TurboRFP 553 573
VIC 530 550
Vybrant DyeCycle Orange 519 563

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

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