This 4-filter "Pinkel" set is perfectly optimized for imaging Cy3 and Cy5 with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is comprised of two single-band exciters along with a dual-band beamsplitter and a dual-band emitter, and thus works best with an exciter filter wheel or slider.
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 5.0 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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6-carboxyrhodamine 6G | 525 | 555 |
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6-JOE | 520 | 548 |
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Alexa Fluor® 514 | 518 | 543 |
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Alexa Fluor® 532 | 534 | 553 |
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Alexa Fluor® 546 | 556 | 572 |
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Alexa Fluor® 555 | 553 | 565 |
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Alexa Fluor® 633 | 631 | 647 |
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Alexa Fluor® 647 | 653 | 668 |
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Alexa Fluor® 660 | 663 | 691 |
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Alexa Fluor® 680 | 679 | 702 |
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Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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APC | 651 | 660 |
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APC (Allophycocyanin) | 651 | 660 |
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ATTO 520 | 516 | 538 |
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ATTO 532 | 501 | 545 |
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ATTO 550 | 553 | 576 |
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ATTO 565 | 563 | 592 |
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ATTO 610 | 614 | 634 |
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ATTO 620 | 618 | 642 |
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ATTO 633 | 629 | 658 |
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ATTO 635 | 635 | 658 |
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ATTO 647 | 644 | 670 |
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ATTO 647N | 645 | 668 |
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ATTO 655 | 663 | 683 |
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ATTO 680 | 680 | 700 |
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BODIPY 650/665-X | 646 | 664 |
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BODIPY TMR-X | 544 | 573 |
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BODIPY TR-X (MeOH) | 544 | 573 |
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Calcium Orange | 589 | 609 |
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Carboxynaphthofluorescein | 599 | 674 |
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Cy3.5™ | 578 | 591 |
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Cy3™ | 554 | 566 |
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Cy5.5™ | 672 | 690 |
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Cy5™ | 649 | 666 |
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DDAO | 648 | 657 |
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Dendra2 (Red) | 554 | 569 |
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DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
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DiI | 551 | 569 |
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DsRed | 559 | 583 |
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DsRed-Express | 556 | 584 |
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dTomato | 556 | 582 |
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DY-530 | 537 | 560 |
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DY-630 | 634 | 656 |
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DyLight 549 | 553 | 569 |
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DyLight 649 | 651 | 673 |
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DyLight 680 | 676 | 705 |
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Eosin | 525 | 546 |
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Ethidium bromide | 518 | 603 |
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Ethidium homodimer | 527 | 617 |
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FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
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FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
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FluoSpheres Orange fluorescent microspheres | 540 | 559 |
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Gold 525 | 521 | 551 |
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Gold 550 | 551 | 564 |
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HCS CellMask Red cytoplasmic | 622 | 644 |
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HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
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HiLyte Fluor™ 555 | 550 | 566 |
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HiLyte Fluor™ 647 | 649 | 673 |
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KFP-Red | 575 | 599 |
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LDS 751 | 560 | 711 |
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LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
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LOLO-1 | 568 | 580 |
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Magnesium Orange | 550 | 574 |
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mApple | 566 | 594 |
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Merocyanine 540 | 559 | 579 |
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MitoTracker™ Deep Red | 641 | 662 |
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MitoTracker™ Orange | 551 | 575 |
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mKO | 549 | 559 |
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mOrange | 546 | 562 |
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mOrange2 | 551 | 565 |
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mRFP | 555 | 583 |
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mRuby | 558 | 605 |
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mStrawberry | 574 | 596 |
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mTangerine | 568 | 585 |
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Nile blue-EtOH | 631 | 660 |
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Nile red-triglyceride | 510 | 583 |
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OFP | 547 | 567 |
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Orange 552 | 552 | 574 |
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pHrodo™, succinimidyl ester | 560 | 587 |
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POPO-3 | 533 | 574 |
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PO-PRO-3 | 539 | 570 |
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Pro-Q Diamond | 556 | 583 |
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Qdot® 565 Nanocrystals | 300 | 564 |
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Qdot® 585 Nanocrystals | 300 | 588 |
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Qdot® 705 Nanocrystals | 300 | 702 |
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Red 650 | 649 | 665 |
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Resorufin | 571 | 584 |
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Rhod-2 | 553 | 577 |
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Rhodamine phalloidin | 558 | 575 |
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R-phycoerythrin (PE) | 565 | 576 |
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SNARF-1 488nm (ph 6.0) | 548 | 586 |
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SNARF-1 514nm (ph 6.0) | 549 | 587 |
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SpectrumFRed (Far Red) | 650 | 673 |
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SpectrumGold | 530 | 556 |
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SpectrumOrange | 554 | 587 |
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SYTO 59 | 622 | 644 |
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SYTO 60 | 650 | 681 |
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SYTO 61 | 618 | 650 |
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SYTO 82 | 540 | 559 |
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SYTOX Orange | 547 | 570 |
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SYTOX Red-DNA | 640 | 658 |
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Tetramethylrhodamine dextran | 554 | 582 |
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TO-PRO-1 | 515 | 531 |
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TO-PRO-3 | 642 | 657 |
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TOTO-1 | 514 | 531 |
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TOTO-3 | 642 | 661 |
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TRITC (Tetramethylrhodamine) | 552 | 578 |
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TurboRFP | 553 | 573 |
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VIC | 530 | 550 |
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Vybrant DyeCycle Orange | 519 | 563 |
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