This 4-filter "Pinkel" set is perfectly optimized for imaging Cy3 and Cy5 with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is comprised of two single-band exciters along with a dual-band beamsplitter and a dual-band emitter, and thus works best with an exciter filter wheel or slider.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,114.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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Clear selection
Select all 4 products from all pages.
FF01-577/690-25
FL-004347 / 0
- Tavg > 90% 565 – 589 nm & Tavg > 90% 665 – 715 nm
- Center Wavelengths of 577 nm & 690 nm and GMBWs of 24 nm & 50 nm
FF02-534/30-25
FL-004433 / 0
- Tavg > 90% 519.5 – 549.5 nm
- Center Wavelength of 534.5 nm and GMBW of 30 nm
- FWHM of 37.7 nm
FF02-628/40-25
FL-004435 / 0
- Tavg > 93% 608 – 648 nm
- Center Wavelength of 628 nm and GMBW of 40 nm
- FWHM of 46.5 nm
FF560/659-Di01-25x36
FL-006924 / 0
- Dichroic Beamsplitter with edges at 560 nm & 659 nm
- Ravg > 95% 514 – 553 nm & Ravg > 95% 617 – 652 nm
- Tavg > 90% 564 – 591 nm & Tavg > 90% 665 – 718 nm
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 6-JOE | 520 | 548 |
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| Alexa Fluor® 514 | 518 | 543 |
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| Alexa Fluor® 532 | 534 | 553 |
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| Alexa Fluor® 546 | 556 | 572 |
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| Alexa Fluor® 555 | 553 | 565 |
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| Alexa Fluor® 633 | 631 | 647 |
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| Alexa Fluor® 647 | 653 | 668 |
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| Alexa Fluor® 660 | 663 | 691 |
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| Alexa Fluor® 680 | 679 | 702 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| APC | 651 | 660 |
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| APC (Allophycocyanin) | 651 | 660 |
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| ATTO 520 | 516 | 538 |
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| ATTO 532 | 501 | 545 |
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| ATTO 550 | 553 | 576 |
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| ATTO 565 | 563 | 592 |
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| ATTO 610 | 614 | 634 |
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| ATTO 620 | 618 | 642 |
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| ATTO 633 | 629 | 658 |
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| ATTO 635 | 635 | 658 |
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| ATTO 647 | 644 | 670 |
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| ATTO 647N | 645 | 668 |
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| ATTO 655 | 663 | 683 |
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| ATTO 680 | 680 | 700 |
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| BODIPY 650/665-X | 646 | 664 |
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| BODIPY TMR-X | 544 | 573 |
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| BODIPY TR-X (MeOH) | 544 | 573 |
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| Calcium Orange | 589 | 609 |
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| Carboxynaphthofluorescein | 599 | 674 |
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| Cy3.5™ | 578 | 591 |
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| Cy3™ | 554 | 566 |
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| Cy5.5™ | 672 | 690 |
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| Cy5™ | 649 | 666 |
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| DDAO | 648 | 657 |
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| Dendra2 (Red) | 554 | 569 |
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
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| DiI | 551 | 569 |
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| DsRed | 559 | 583 |
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| DsRed-Express | 556 | 584 |
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| dTomato | 556 | 582 |
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| DY-530 | 537 | 560 |
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| DY-630 | 634 | 656 |
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| DyLight 549 | 553 | 569 |
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| DyLight 649 | 651 | 673 |
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| DyLight 680 | 676 | 705 |
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| Eosin | 525 | 546 |
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| Ethidium bromide | 518 | 603 |
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| Ethidium homodimer | 527 | 617 |
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
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| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
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| Gold 525 | 521 | 551 |
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| Gold 550 | 551 | 564 |
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| HCS CellMask Red cytoplasmic | 622 | 644 |
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
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| HiLyte Fluor™ 555 | 550 | 566 |
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| HiLyte Fluor™ 647 | 649 | 673 |
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| KFP-Red | 575 | 599 |
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| LDS 751 | 560 | 711 |
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
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| LOLO-1 | 568 | 580 |
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| Magnesium Orange | 550 | 574 |
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| mApple | 566 | 594 |
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| Merocyanine 540 | 559 | 579 |
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| MitoTracker™ Deep Red | 641 | 662 |
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| MitoTracker™ Orange | 551 | 575 |
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| mKO | 549 | 559 |
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| mOrange | 546 | 562 |
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| mOrange2 | 551 | 565 |
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| mRFP | 555 | 583 |
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| mRuby | 558 | 605 |
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| mStrawberry | 574 | 596 |
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| mTangerine | 568 | 585 |
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| Nile blue-EtOH | 631 | 660 |
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| Nile red-triglyceride | 510 | 583 |
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| OFP | 547 | 567 |
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| Orange 552 | 552 | 574 |
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| pHrodo™, succinimidyl ester | 560 | 587 |
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| POPO-3 | 533 | 574 |
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| PO-PRO-3 | 539 | 570 |
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| Pro-Q Diamond | 556 | 583 |
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| Qdot® 565 Nanocrystals | 300 | 564 |
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| Qdot® 585 Nanocrystals | 300 | 588 |
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| Red 650 | 649 | 665 |
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| Resorufin | 571 | 584 |
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| Rhod-2 | 553 | 577 |
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| Rhodamine phalloidin | 558 | 575 |
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| R-phycoerythrin (PE) | 565 | 576 |
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
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| SpectrumFRed (Far Red) | 650 | 673 |
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| SpectrumGold | 530 | 556 |
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| SpectrumOrange | 554 | 587 |
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| SYTO 59 | 622 | 644 |
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| SYTO 60 | 650 | 681 |
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| SYTO 61 | 618 | 650 |
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| SYTO 82 | 540 | 559 |
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| SYTOX Orange | 547 | 570 |
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| SYTOX Red-DNA | 640 | 658 |
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| Tetramethylrhodamine dextran | 554 | 582 |
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| TO-PRO-1 | 515 | 531 |
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| TO-PRO-3 | 642 | 657 |
|
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| TOTO-1 | 514 | 531 |
|
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| TOTO-3 | 642 | 661 |
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
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| TurboRFP | 553 | 573 |
|
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| VIC | 530 | 550 |
|
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| Vybrant DyeCycle Orange | 519 | 563 |
|
total selected
Clear selection
Select all 4 products from all pages.
FF01-577/690-25
FL-004347 / 0
- Tavg > 90% 565 – 589 nm & Tavg > 90% 665 – 715 nm
- Center Wavelengths of 577 nm & 690 nm and GMBWs of 24 nm & 50 nm
FF02-534/30-25
FL-004433 / 0
- Tavg > 90% 519.5 – 549.5 nm
- Center Wavelength of 534.5 nm and GMBW of 30 nm
- FWHM of 37.7 nm