This 5-filter "Sedat" set is ideal for high-speed sequential imaging Cy3 and Cy5 with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is comprised of two single-band exciters and two single-band emitters, along with a dual-band beamsplitter, and thus works best when used with both exciter and emitter filter wheels.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,374.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-534/30-25
FL-004433 / 0
- Tavg > 90% 519.5 – 549.5 nm
- Center Wavelength of 534.5 nm and GMBW of 30 nm
- FWHM of 37.7 nm
FF02-628/40-25
FL-004435 / 0
- Tavg > 93% 608 – 648 nm
- Center Wavelength of 628 nm and GMBW of 40 nm
- FWHM of 46.5 nm
FF01-585/40-25
FL-004494 / 0
- Tavg > 90% 565 – 605 nm
- Center Wavelength of 585 nm and GMBW of 40 nm
- FWHM of 46.2 nm
FF01-692/40-25
FL-004702 / 0
- Tavg > 93% 672 – 712 nm
- Center Wavelength of 692 nm and GMBW of 40 nm
- FWHM of 47.6 nm
FF560/659-Di01-25x36
FL-006924 / 0
- Dichroic Beamsplitter with edges at 560 nm & 659 nm
- Ravg > 95% 514 – 553 nm & Ravg > 95% 617 – 652 nm
- Tavg > 90% 564 – 591 nm & Tavg > 90% 665 – 718 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 6-JOE | 520 | 548 |
|
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| Alexa Fluor® 514 | 518 | 543 |
|
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| Alexa Fluor® 532 | 534 | 553 |
|
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| Alexa Fluor® 546 | 556 | 572 |
|
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| Alexa Fluor® 555 | 553 | 565 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 647 | 653 | 668 |
|
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| Alexa Fluor® 660 | 663 | 691 |
|
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| Alexa Fluor® 680 | 679 | 702 |
|
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| APC | 651 | 660 |
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| APC (Allophycocyanin) | 651 | 660 |
|
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| AsRed 2 | 576 | 592 |
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| ATTO 532 | 501 | 545 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 620 | 618 | 642 |
|
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| ATTO 633 | 629 | 658 |
|
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| ATTO 635 | 635 | 658 |
|
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| ATTO 647 | 644 | 670 |
|
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| ATTO 647N | 645 | 668 |
|
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| ATTO 655 | 663 | 683 |
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| ATTO 680 | 680 | 700 |
|
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY 650/665-X | 646 | 664 |
|
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| BODIPY TMR-X | 544 | 573 |
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
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| Calcium Orange | 589 | 609 |
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| Cy3.5™ | 578 | 591 |
|
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| Cy3™ | 554 | 566 |
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| Cy5.5™ | 672 | 690 |
|
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| Cy5™ | 649 | 666 |
|
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| DDAO | 648 | 657 |
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| Dendra2 (Red) | 554 | 569 |
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
|
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| DiI | 551 | 569 |
|
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| DsRed | 559 | 583 |
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| DsRed-Express | 556 | 584 |
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| dTomato | 556 | 582 |
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| DY-530 | 537 | 560 |
|
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| DY-630 | 634 | 656 |
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| DyLight 549 | 553 | 569 |
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| DyLight 649 | 651 | 673 |
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| DyLight 680 | 676 | 705 |
|
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| Eosin | 525 | 546 |
|
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| Ethidium bromide | 518 | 603 |
|
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| Ethidium homodimer | 527 | 617 |
|
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
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| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
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| Gold 525 | 521 | 551 |
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| Gold 550 | 551 | 564 |
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| HCS CellMask Red cytoplasmic | 622 | 644 |
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| HiLyte Fluor™ 555 | 550 | 566 |
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| HiLyte Fluor™ 647 | 649 | 673 |
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| KFP-Red | 575 | 599 |
|
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| LDS 751 | 560 | 711 |
|
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
|
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| LOLO-1 | 568 | 580 |
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| LysoTracker Red | 573 | 592 |
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| Magnesium Orange | 550 | 574 |
|
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| mApple | 566 | 594 |
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| Merocyanine 540 | 559 | 579 |
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| MitoTracker™ Deep Red | 641 | 662 |
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| MitoTracker™ Orange | 551 | 575 |
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| mKO | 549 | 559 |
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| mOrange | 546 | 562 |
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| mOrange2 | 551 | 565 |
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| mRFP | 555 | 583 |
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| mRuby | 558 | 605 |
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| mStrawberry | 574 | 596 |
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| mTangerine | 568 | 585 |
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| Nile blue-EtOH | 631 | 660 |
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| Nile red-triglyceride | 510 | 583 |
|
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| OFP | 547 | 567 |
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| Orange 552 | 552 | 574 |
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| pHrodo™, succinimidyl ester | 560 | 587 |
|
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| POPO-3 | 533 | 574 |
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| PO-PRO-3 | 539 | 570 |
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| Propidium Iodide (PI) | 305 | 617 |
|
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| Pro-Q Diamond | 556 | 583 |
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| Qdot® 565 Nanocrystals | 300 | 564 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| ReAsH-CCPGCC | 592 | 606 |
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| Red 650 | 649 | 665 |
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| Resorufin | 571 | 584 |
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| Rhod-2 | 553 | 577 |
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| Rhodamine phalloidin | 558 | 575 |
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| R-phycoerythrin (PE) | 565 | 576 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
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| SpectrumFRed (Far Red) | 650 | 673 |
|
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| SpectrumGold | 530 | 556 |
|
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| SpectrumOrange | 554 | 587 |
|
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| SYTO 59 | 622 | 644 |
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| SYTO 60 | 650 | 681 |
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| SYTO 61 | 618 | 650 |
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| SYTO 82 | 540 | 559 |
|
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| SYTOX Orange | 547 | 570 |
|
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| SYTOX Red-DNA | 640 | 658 |
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| Tetramethylrhodamine dextran | 554 | 582 |
|
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| TO-PRO-1 | 515 | 531 |
|
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| TO-PRO-3 | 642 | 657 |
|
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| TOTO-3 | 642 | 661 |
|
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
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| TurboRFP | 553 | 573 |
|
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| VIC | 530 | 550 |
|
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| Vybrant DyeCycle Orange | 519 | 563 |
|
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