BrightLine Basic™ single-band filter set, optimized for Cy3.5 and other like fluorophores

Semrock Part Number: Cy3.5-A-Basic-NQF

IDEX Part Number: S-001267  /

Semrock

This filter set is ideal for use with Cy3.5. It is also ideal for some of the new flavors of monomeric red fluorescent proteins, including mStrawberry. This hard-coated filter set is the best value on the market, delivering substantially higher brightness and contrast than the comparable premium soft-coated filter sets.

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  • 1,324.00 USD Each
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FF01-565/24-25

FL-004554 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 553 – 577 nm
  • Center Wavelength of 565 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FF01-620/52-25

FL-004561 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 594 – 646 nm
  • Center Wavelength of 620 nm and GMBW of 52 nm
  • FWHM of 58.2 nm

FF585-Di01-25x36

FL-007096 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 585 nm
  • Ravg > 90% 533 – 580 nm
  • Tavg > 90% 595 – 800 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)
1.1 mm
(Can accept a 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
ATTO 594 601 624
BOBO™-3 570 605
BODIPY TR-X phallacidin 590 621
Calcium Crimson 589 609
Calcium Orange 589 609
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
Cy3.5™ 578 591
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-590 582 599
DyLight 594 593 618
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Red fluorescent microspheres 577 607
HcRed1 588 618
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 594 592 616
Hypericin 600 603
KFP-Red 575 599
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
LOLO-1 568 580
LysoTracker Red 573 592
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
Merocyanine 540 559 579
MitoTracker™ Orange 551 575
MitoTracker™ Red 578 598
mKate2 588 633
mPlum 589 649
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile red-phospolipid 553 637
Orange 552 552 574
pHrodo™, succinimidyl ester 560 587
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
ReAsH-CCPGCC 592 606
Red 580 580 603
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
SpectrumOrange 554 587
SpectrumRed 587 615
Sulforhodamine 101-EtOH 578 593
Tetramethylrhodamine dextran 554 582
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TRITC (Tetramethylrhodamine) 552 578
TurboFP635(Katushka) 591 638
TurboRFP 553 573
X-Rhod-1 Indicator 580 601
YOYO-3 611 631

Related Products

FF01-565/24-25

FL-004554 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 553 – 577 nm
  • Center Wavelength of 565 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FF01-620/52-25

FL-004561 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 594 – 646 nm
  • Center Wavelength of 620 nm and GMBW of 52 nm
  • FWHM of 58.2 nm

FF585-Di01-25x36

FL-007096 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 585 nm
  • Ravg > 90% 533 – 580 nm
  • Tavg > 90% 595 – 800 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)
1.1 mm
(Can accept a 2 mm dichroic)