This filter set is ideal for use with Cy3.5. It is also ideal for some of the new flavors of monomeric red fluorescent proteins, including mStrawberry. This hard-coated filter set is the best value on the market, delivering substantially higher brightness and contrast than the comparable premium soft-coated filter sets.
FL-004554 / 0
FL-004561 / 0
FL-007096 / 0
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 21.8 mm | 5.0 mm |
Emitter (Diameter) | 23.3 mm | 5.0 mm |
Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |