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BrightLine® 2.0 single-band filter set, optimized for Cy3 and other like fluorophores

Semrock Part Number: Cy3-4040D-000

IDEX Part Number: S-445518  /

Semrock

BrightLine™ 2.0 filter sets introduces a unified, improved design that delivers enhanced contrast and leverages the latest advances in coating technologies across this industry-leading filter family. The fluorescence bandpass filters and sets are meticulously optimized for seamless integration across a wide range of fluorescence instruments. With its all-thin-film, hard-coated construction, BrightLine 2.0 sets a new standard in performance and reliability.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 908.43 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FF01-533/44-25

FL-445516

Each
  • Tavg > 95% 511 – 555 nm
  • Center Wavelength of 533 nm and GMBW of 44 nm
  • FWHM of 50.2 nm

FL-445516

FF02-593/40-25

FL-442965

Each
  • Tavg > 95% 573 – 613 nm
  • Center Wavelength of 593 nm and GMBW of 40 nm
  • FWHM of 45.9 nm

FL-442965

FF564-Di02-25x36

FL-443965

Each
  • Dichroic Beamsplitter with edge at 563.5 nm
  • Ravg > 98% 350 – 554 nm
  • Tavg > 95% 573 – 1100 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-443965

Optical Specifications Value
Blocking BrightLine® 2.0 filters have blocking far exceeding OD 8 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Exciter/Emitter Orientation Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation Reflective surface marked with part number - Orient in direction of incoming light
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
6-JOE 520 548
Alexa Fluor® 532 534 553
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 532 501 545
ATTO 550 553 576
ATTO 565 563 592
BOBO™-3 570 605
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
Calcium Orange 589 609
Cy3.5™ 578 591
Cy3™ 554 566
Dendra2 (Red) 554 569
DiI 551 569
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-530 537 560
DyLight 549 553 569
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Orange fluorescent microspheres 540 559
FluoSpheres Red fluorescent microspheres 577 607
Gold 550 551 564
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 555 550 566
JC-1 499 525
KFP-Red 575 599
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
Merocyanine 540 559 579
MitoTracker™ Orange 551 575
mKO 549 559
mOrange 546 562
mOrange2 551 565
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
pHrodo™, succinimidyl ester 560 587
Phycoerythrin (PE) 565 575
POPO-3 533 574
PO-PRO-3 539 570
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
R-phycoerythrin (PE) 565 576
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 514nm (ph 6.0) 549 587
SpectrumGold 530 556
SpectrumOrange 554 587
SYTO 82 540 559
SYTOX Orange 547 570
Tetramethylrhodamine dextran 554 582
Texas Red DHPE 584 608
TO-PRO-1 515 531
TOTO-1 514 531
TRITC (Tetramethylrhodamine) 552 578
TurboRFP 553 573
Vybrant DyeCycle Orange 519 563

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

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