BrightLine® single-band filter set, optimized for Cy3 and other like fluorophores

Semrock Part Number: Cy3-4040C-OFX

IDEX Part Number: S-001265  /

Semrock

This filter set is ideal for use with Cy3™ and a variety of other orange fluorophores (including DsRed fluorescent protein). It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.
Are you co-labeling with YFP, GFP, or FITC? In order to substantially eliminate green-fluorophore bleedthrough we recommend using our TRITC-B filter set. For mercury (e.g., HBO) lamp users labeling with Cy3 the single-fluorophore signal-to-noise ratio will be slightly higher, and the brightness slightly lower, with the TRITC-A filter set. Xenon lamp users of the TRITC-A filter set who are labeling with Cy3 will enjoy a significantly enhanced signal-to-noise ratio and reduced photobleaching in exchange for brightness.

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  • Price Not Available.
  • 1,764.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter Role Filter Size Maximum Thickness
Exciter (Diameter)32.0 mm5.0 mm
Emitter (Diameter)32.0 mm3.5 mm
Dichroic (L × W)32.0 mm × 44.0 mm
(Corners must me cut for OFX cube)
1.5 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
6-JOE 520 548
Alexa Fluor® 532 534 553
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 532 501 545
ATTO 550 553 576
ATTO 565 563 592
BOBO™-3 570 605
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
Calcium Orange 589 609
Cy3.5™ 578 591
Cy3™ 554 566
Dendra2 (Red) 554 569
DiI 551 569
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-530 537 560
DyLight 549 553 569
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Orange fluorescent microspheres 540 559
FluoSpheres Red fluorescent microspheres 577 607
Gold 550 551 564
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 555 550 566
JC-1 499 525
KFP-Red 575 599
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
Merocyanine 540 559 579
MitoTracker™ Orange 551 575
mKO 549 559
mOrange 546 562
mOrange2 551 565
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
pHrodo™, succinimidyl ester 560 587
Phycoerythrin (PE) 565 575
POPO-3 533 574
PO-PRO-3 539 570
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
R-phycoerythrin (PE) 565 576
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 514nm (ph 6.0) 549 587
SpectrumGold 530 556
SpectrumOrange 554 587
SYTO 82 540 559
SYTOX Orange 547 570
Tetramethylrhodamine dextran 554 582
Texas Red DHPE 584 608
TO-PRO-1 515 531
TOTO-1 514 531
TRITC (Tetramethylrhodamine) 552 578
TurboRFP 553 573
Vybrant DyeCycle Orange 519 563

Standard Component Sizes

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)32.0 mm5.0 mm
Emitter (Diameter)32.0 mm3.5 mm
Dichroic (L × W)32.0 mm × 44.0 mm
(Corners must me cut for OFX cube)
1.5 mm