This filter set is ideal for use with Cy3™ and a variety of other orange fluorophores (including DsRed fluorescent protein). It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.
Are you co-labeling with YFP, GFP, or FITC? In order to substantially eliminate green-fluorophore bleedthrough we recommend using our TRITC-B filter set. For mercury (e.g., HBO) lamp users labeling with Cy3 the single-fluorophore signal-to-noise ratio will be slightly higher, and the brightness slightly lower, with the TRITC-A filter set. Xenon lamp users of the TRITC-A filter set who are labeling with Cy3 will enjoy a significantly enhanced signal-to-noise ratio and reduced photobleaching in exchange for brightness.
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Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 32.0 mm | 5.0 mm |
Emitter (Diameter) | 32.0 mm | 3.5 mm |
Dichroic (L × W) | 32.0 mm × 44.0 mm (Corners must me cut for OFX cube) | 1.5 mm |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
---|---|---|---|---|
2-dodecylresorufin-lipid | 582 | 595 |
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5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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6-JOE | 520 | 548 |
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Alexa Fluor® 532 | 534 | 553 |
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Alexa Fluor® 546 | 556 | 572 |
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Alexa Fluor® 555 | 553 | 565 |
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Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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AsRed 2 | 576 | 592 |
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ATTO 532 | 501 | 545 |
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ATTO 550 | 553 | 576 |
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ATTO 565 | 563 | 592 |
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BOBOâ„¢-3 | 570 | 605 |
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BODIPY TMR-X | 544 | 573 |
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BODIPY TR-X (MeOH) | 544 | 573 |
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Calcium Orange | 589 | 609 |
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Cy3.5â„¢ | 578 | 591 |
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Cy3â„¢ | 554 | 566 |
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Dendra2 (Red) | 554 | 569 |
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DiI | 551 | 569 |
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DsRed | 559 | 583 |
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DsRed-Express | 556 | 584 |
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dTomato | 556 | 582 |
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DY-530 | 537 | 560 |
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DyLight 549 | 553 | 569 |
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Ethidium bromide | 518 | 603 |
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Ethidium homodimer | 527 | 617 |
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FluoSpheres Orange fluorescent microspheres | 540 | 559 |
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FluoSpheres Red fluorescent microspheres | 577 | 607 |
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Gold 550 | 551 | 564 |
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HCS LipidTOX Red phospholipidosis | 584 | 608 |
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HiLyte Fluorâ„¢ 555 | 550 | 566 |
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JC-1 | 499 | 525 |
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KFP-Red | 575 | 599 |
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LysoTracker Red | 573 | 592 |
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LysoTracker Yellow HCK-123 | 488 | 565 |
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Magnesium Orange | 550 | 574 |
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mApple | 566 | 594 |
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mCherry | 587 | 610 |
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Merocyanine 540 | 559 | 579 |
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MitoTrackerâ„¢ Orange | 551 | 575 |
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mKO | 549 | 559 |
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mOrange | 546 | 562 |
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mOrange2 | 551 | 565 |
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mRFP | 555 | 583 |
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mRFP1 | 584 | 700 |
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mRuby | 558 | 605 |
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mStrawberry | 574 | 596 |
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mTangerine | 568 | 585 |
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Nile red-triglyceride | 510 | 583 |
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OFP | 547 | 567 |
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Orange 552 | 552 | 574 |
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pHrodoâ„¢, succinimidyl ester | 560 | 587 |
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Phycoerythrin (PE) | 565 | 575 |
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POPO-3 | 533 | 574 |
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PO-PRO-3 | 539 | 570 |
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Propidium Iodide (PI) | 305 | 617 |
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Pro-Q Diamond | 556 | 583 |
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Qdot® 585 Nanocrystals | 300 | 588 |
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Qdot® 605 Nanocrystals | 300 | 602 |
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Resorufin | 571 | 584 |
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Rhod-2 | 553 | 577 |
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Rhodamine phalloidin | 558 | 575 |
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R-phycoerythrin (PE) | 565 | 576 |
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SNARF-1 488nm (ph 6.0) | 548 | 586 |
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SNARF-1 514nm (ph 6.0) | 549 | 587 |
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SpectrumGold | 530 | 556 |
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SpectrumOrange | 554 | 587 |
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SYTO 82 | 540 | 559 |
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SYTOX Orange | 547 | 570 |
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Tetramethylrhodamine dextran | 554 | 582 |
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Texas Red DHPE | 584 | 608 |
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TO-PRO-1 | 515 | 531 |
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TOTO-1 | 514 | 531 |
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TRITC (Tetramethylrhodamine) | 552 | 578 |
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TurboRFP | 553 | 573 |
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Vybrant DyeCycle Orange | 519 | 563 |
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