This filter set is ideal for use with Cy3™ and a variety of other orange fluorophores (including DsRed fluorescent protein). It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.
Are you co-labeling with YFP, GFP, or FITC? In order to substantially eliminate green-fluorophore bleedthrough we recommend using our TRITC-B filter set. For mercury (e.g., HBO) lamp users labeling with Cy3 the single-fluorophore signal-to-noise ratio will be slightly higher, and the brightness slightly lower, with the TRITC-A filter set. Xenon lamp users of the TRITC-A filter set who are labeling with Cy3 will enjoy a significantly enhanced signal-to-noise ratio and reduced photobleaching in exchange for brightness.
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This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||25.0 mm||5.0 mm|
|Emitter (Diameter)||25.0 mm||5.0 mm|
|Dichroic (L × W)||25.2 mm × 35.6 mm||1.5 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|
|What is Pixel Shift, and how does the Semrock ZERO™ option help?|