This filter set is ideal for use with Cy3™ and a variety of other orange fluorophores (including DsRed fluorescent protein). It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.
Are you co-labeling with YFP, GFP, or FITC? In order to substantially eliminate green-fluorophore bleedthrough we recommend using our TRITC-B filter set. For mercury (e.g., HBO) lamp users labeling with Cy3 the single-fluorophore signal-to-noise ratio will be slightly higher, and the brightness slightly lower, with the TRITC-A filter set. Xenon lamp users of the TRITC-A filter set who are labeling with Cy3 will enjoy a significantly enhanced signal-to-noise ratio and reduced photobleaching in exchange for brightness.
FL-004550 / 0
FL-004557 / 0
FL-007122 / 0
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 21.8 mm | 5.0 mm |
Emitter (Diameter) | 23.3 mm | 5.0 mm |
Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
Topic | Description | |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters | |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters | |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD | |
What is Pixel Shift, and how does the Semrock ZERO™ option help? |
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