This filter set is ideal for use with Cy3™ and a variety of other orange fluorophores (including DsRed fluorescent protein). It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.
Are you co-labeling with YFP, GFP, or FITC? In order to substantially eliminate green-fluorophore bleedthrough we recommend using our TRITC-B filter set. For mercury (e.g., HBO) lamp users labeling with Cy3 the single-fluorophore signal-to-noise ratio will be slightly higher, and the brightness slightly lower, with the TRITC-A filter set. Xenon lamp users of the TRITC-A filter set who are labeling with Cy3 will enjoy a significantly enhanced signal-to-noise ratio and reduced photobleaching in exchange for brightness.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,553.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-531/40-25
FL-004550 / 0
- Tavg > 93% 511 – 551 nm
- Center Wavelength of 531 nm and GMBW of 40 nm
- FWHM of 45.8 nm
FF01-593/40-25
FL-004557 / 0
- Tavg > 93% 573 – 613 nm
- Center Wavelength of 593 nm and GMBW of 40 nm
- FWHM of 45.7 nm
FF562-Di03-25x36
FL-007122 / 0
- Dichroic Beamsplitter with edge at 562 nm
- Ravg > 98% 350 – 554.5 nm
- Tavg > 93% 569.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
| Topic | Description | |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters | |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters | |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD | |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? |
|
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
|
| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
|
| 6-JOE | 520 | 548 |
|
|
| Alexa Fluor® 532 | 534 | 553 |
|
|
| Alexa Fluor® 546 | 556 | 572 |
|
|
| Alexa Fluor® 555 | 553 | 565 |
|
|
| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
|
| AsRed 2 | 576 | 592 |
|
|
| ATTO 532 | 501 | 545 |
|
|
| ATTO 550 | 553 | 576 |
|
|
| ATTO 565 | 563 | 592 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY TMR-X | 544 | 573 |
|
|
| BODIPY TR-X (MeOH) | 544 | 573 |
|
|
| Calcium Orange | 589 | 609 |
|
|
| Cy3.5™ | 578 | 591 |
|
|
| Cy3™ | 554 | 566 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
|
| DiI | 551 | 569 |
|
|
| DsRed | 559 | 583 |
|
|
| DsRed-Express | 556 | 584 |
|
|
| dTomato | 556 | 582 |
|
|
| DY-530 | 537 | 560 |
|
|
| DyLight 549 | 553 | 569 |
|
|
| Ethidium bromide | 518 | 603 |
|
|
| Ethidium homodimer | 527 | 617 |
|
|
| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| Gold 550 | 551 | 564 |
|
|
| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
|
| HiLyte Fluor™ 555 | 550 | 566 |
|
|
| JC-1 | 499 | 525 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| LysoTracker Yellow HCK-123 | 488 | 565 |
|
|
| Magnesium Orange | 550 | 574 |
|
|
| mApple | 566 | 594 |
|
|
| mCherry | 587 | 610 |
|
|
| Merocyanine 540 | 559 | 579 |
|
|
| MitoTracker™ Orange | 551 | 575 |
|
|
| mKO | 549 | 559 |
|
|
| mOrange | 546 | 562 |
|
|
| mOrange2 | 551 | 565 |
|
|
| mRFP | 555 | 583 |
|
|
| mRFP1 | 584 | 700 |
|
|
| mRuby | 558 | 605 |
|
|
| mStrawberry | 574 | 596 |
|
|
| mTangerine | 568 | 585 |
|
|
| Nile red-triglyceride | 510 | 583 |
|
|
| OFP | 547 | 567 |
|
|
| Orange 552 | 552 | 574 |
|
|
| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| Phycoerythrin (PE) | 565 | 575 |
|
|
| POPO-3 | 533 | 574 |
|
|
| PO-PRO-3 | 539 | 570 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Pro-Q Diamond | 556 | 583 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
|
| Rhodamine phalloidin | 558 | 575 |
|
|
| R-phycoerythrin (PE) | 565 | 576 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| SpectrumGold | 530 | 556 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| SYTO 82 | 540 | 559 |
|
|
| SYTOX Orange | 547 | 570 |
|
|
| Tetramethylrhodamine dextran | 554 | 582 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| TO-PRO-1 | 515 | 531 |
|
|
| TOTO-1 | 514 | 531 |
|
|
| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
|
| TurboRFP | 553 | 573 |
|
|
| Vybrant DyeCycle Orange | 519 | 563 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-531/40-25
FL-004550 / 0
- Tavg > 93% 511 – 551 nm
- Center Wavelength of 531 nm and GMBW of 40 nm
- FWHM of 45.8 nm
FF01-593/40-25
FL-004557 / 0
- Tavg > 93% 573 – 613 nm
- Center Wavelength of 593 nm and GMBW of 40 nm
- FWHM of 45.7 nm
FF562-Di03-25x36
FL-007122 / 0
- Dichroic Beamsplitter with edge at 562 nm
- Ravg > 98% 350 – 554.5 nm
- Tavg > 93% 569.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
| Topic | Description | |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters | |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters | |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD | |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? |
|
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