BrightLine® single-band filter set, optimized for Cyan Fluorescent Protein and other like fluorophores

Semrock Part Number: CFP-2432C-NTE-ZERO

IDEX Part Number: S-000063  /

Semrock

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring CFP and its variants – no more compromising or exchanging filter sets for different CFP experiments. For CFP-YFP FRET measurements, consider our economical FRET combination set FRET-CFP/YFP-A, or our high-performance "Sedat" filter set CFP/YFP-2X2M-A.

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  • 1,573.00 USD Each
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FF01-483/32-25

FL-004598 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 467 – 499 nm
  • Center Wavelength of 483 nm and GMBW of 32 nm
  • FWHM of 38.2 nm

FF02-438/24-25

FL-004649 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FF458-Di02-25x36

FL-007114 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 458 nm
  • Ravg > 98% 350 – 450 nm
  • Tavg > 93% 467 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Yellow 470 550
AmCyan1 458 489
Aqua 431 430 478
ATTO 425 436 484
ATTO 465 453 507
BD Horizon V500 410 500
BOBO™-1 461 484
Cerulean 434 473
CFP (ECFP) 433 475
DEAC 432 472
DY-415 418 470
ECFP 435 475
evoglow-Bs1 449 496
LysoSensor Green 448 502
mBBr+GSH 394 490
mTFP1 462 492
POPO-1 433 457
PO-PRO-1 433 456
SpectrumAqua 434 481
SYTO 45 451 485
SYTOX Blue 444 470
TagCFP 458 480

Related Products

FF01-483/32-25

FL-004598 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 467 – 499 nm
  • Center Wavelength of 483 nm and GMBW of 32 nm
  • FWHM of 38.2 nm

FF02-438/24-25

FL-004649 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FF458-Di02-25x36

FL-007114 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 458 nm
  • Ravg > 98% 350 – 450 nm
  • Tavg > 93% 467 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.