This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring CFP and its variants – no more compromising or exchanging filter sets for different CFP experiments. For CFP-YFP FRET measurements, consider our economical FRET combination set FRET-CFP/YFP-A, or our high-performance "Sedat" filter set CFP/YFP-2X2M-A.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,485.96 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-483/32-25
FL-004598 / 0
- Tavg > 93% 467 – 499 nm
- Center Wavelength of 483 nm and GMBW of 32 nm
- FWHM of 38.2 nm
FF02-438/24-25
FL-004649 / 0
- Tavg > 93% 426 – 450 nm
- Center Wavelength of 438 nm and GMBW of 24 nm
- FWHM of 29.5 nm
FF458-Di02-25x36
FL-007114 / 0
- Dichroic Beamsplitter with edge at 458 nm
- Ravg > 98% 350 – 450 nm
- Tavg > 93% 467 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Yellow | 470 | 550 |
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| AmCyan1 | 458 | 489 |
|
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| Aqua 431 | 430 | 478 |
|
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| ATTO 425 | 436 | 484 |
|
|
| ATTO 465 | 453 | 507 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| Cerulean | 434 | 473 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| DEAC | 432 | 472 |
|
|
| DY-415 | 418 | 470 |
|
|
| ECFP | 435 | 475 |
|
|
| evoglow-Bs1 | 449 | 496 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| mTFP1 | 462 | 492 |
|
|
| POPO-1 | 433 | 457 |
|
|
| PO-PRO-1 | 433 | 456 |
|
|
| SpectrumAqua | 434 | 481 |
|
|
| SYTO 45 | 451 | 485 |
|
|
| SYTOX Blue | 444 | 470 |
|
|
| TagCFP | 458 | 480 |
|
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-483/32-25
FL-004598 / 0
- Tavg > 93% 467 – 499 nm
- Center Wavelength of 483 nm and GMBW of 32 nm
- FWHM of 38.2 nm
FF02-438/24-25
FL-004649 / 0
- Tavg > 93% 426 – 450 nm
- Center Wavelength of 438 nm and GMBW of 24 nm
- FWHM of 29.5 nm
FF458-Di02-25x36
FL-007114 / 0
- Dichroic Beamsplitter with edge at 458 nm
- Ravg > 98% 350 – 450 nm
- Tavg > 93% 467 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
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