This Full-multiband penta-band set is designed for imaging DAPI, FITC, TRITC, Cy5 and Cy7 (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized to be illuminated with Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths. This set has been designed to deliver significant fluorescence signal improvements for the CELESTA light engine compared with sets designed for standard broadband light sources such as mercury or xenon arc lamps or other LED-based light engines. The complete set is comprised of a penta-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.
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FF01-391/477/549/639/741-25
FL-410455 / 0
- Tavg > 85% 369 – 413 nm, Tavg > 90% 471 – 483 nm, Tavg > 90% 541 – 557 nm, Tavg > 90% 630 – 647 nm & Tavg > 90% 725 – 757 nm
- Center Wavelengths of 391nm, 477 nm, 549 nm, 639 nm & 741 nm and GMBWs of 44 nm, 12 nm, 16 nm, 17 nm & 32 nm
- Optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths
FF01-441/511/593/684/817-25
FL-410484 / 0
- Tavg > 90% 426 – 456 nm, Tavg > 90% 498 – 524 nm, Tavg > 90% 574 – 611 nm, Tavg > 90% 667 – 701 nm & Tavg > 90% 784 – 850 nm
- Center Wavelengths of 441nm, 511 nm, 592.5 nm, 684 nm & 817 nm and GMBWs of 30 nm, 26 nm, 37 nm, 34 nm & 66 nm
- Optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths
FF421/491/567/659/776-Di01-25x36
FL-410491 / 0
- Dichroic Beamsplitter with edges at 421 nm, 491 nm, 567 nm, 659 nm & 776 nm
- Ravg > 95% 369 – 413 nm, Ravg > 95% 471 – 483 nm, Ravg > 95% 541 – 557 nm, Ravg > 95% 630 – 647 nm & Ravg > 95% 725 – 757 nm
- Tavg > 93% 426 – 456 nm, Tavg > 93% 498 – 524 nm, Tavg > 93% 574 – 611 nm, Tavg > 93% 667 – 701 nm & Tavg > 93% 784 – 850 nm
- Optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths
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This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Acridine Yellow | 470 | 550 |
|
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| Alexa Fluor® 405 | 401 | 422 |
|
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| Alexa Fluor® 430 | 431 | 541 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 546 | 556 | 572 |
|
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| Alexa Fluor® 555 | 553 | 565 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 633 | 631 | 647 |
|
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| Alexa Fluor® 647 | 653 | 668 |
|
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| Alexa Fluor® 660 | 663 | 691 |
|
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| Alexa Fluor® 680 | 679 | 702 |
|
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| Alexa Fluor® 700 | 696 | 719 |
|
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| Alexa Fluor® 750 | 751 | 776 |
|
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| Alexa Fluor® 790 | 782 | 805 |
|
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| AmCyan1 | 458 | 489 |
|
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| APC | 651 | 660 |
|
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| APC (Allophycocyanin) | 651 | 660 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 465 | 453 | 507 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 633 | 629 | 658 |
|
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| ATTO 635 | 635 | 658 |
|
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| ATTO 647 | 644 | 670 |
|
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| ATTO 647N | 645 | 668 |
|
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| ATTO 655 | 663 | 683 |
|
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| ATTO 680 | 680 | 700 |
|
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| ATTO 700 | 699 | 719 |
|
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| ATTO 725 | 727 | 754 |
|
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| ATTO 740 | 740 | 764 |
|
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| BD Horizon V450 | 406 | 449 |
|
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| BFP (EBFP) | 380 | 440 |
|
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| BOBO™-1 | 461 | 484 |
|
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY 650/665-X | 646 | 664 |
|
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| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY TMR-X | 544 | 573 |
|
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
|
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| Calcium Orange | 589 | 609 |
|
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| Cascade Blue™ | 401 | 419 |
|
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| CellTrace calcein violet | 400 | 452 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
|
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| Cy3™ | 554 | 566 |
|
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| Cy5.5™ | 672 | 690 |
|
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| Cy5™ | 649 | 666 |
|
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| Cy7™ | 753 | 775 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
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| DAF-FM-NO | 495 | 519 |
|
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| DAPI | 359 | 461 |
|
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| DDAO | 648 | 657 |
|
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| Dendra2 (Green) | 491 | 507 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
|
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| DiI | 551 | 569 |
|
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| DiO | 475 | 500 |
|
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| DiR | 750 | 782 |
|
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| Dronpa | 502 | 516 |
|
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| DsRed | 559 | 583 |
|
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| DsRed-Express | 556 | 584 |
|
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| dTomato | 556 | 582 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
|
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| DY-630 | 634 | 656 |
|
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| DY-635 | 646 | 670 |
|
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| DY-781 | 784 | 802 |
|
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| DyLight 405 | 398 | 420 |
|
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| DyLight 649 | 651 | 673 |
|
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| DyLight 680 | 676 | 705 |
|
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| DyLight 750 | 751 | 778 |
|
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| DyLight 800 | 777 | 794 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
|
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| Ethidium bromide | 518 | 603 |
|
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| Ethidium homodimer | 527 | 617 |
|
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| evoglow-Bs1 | 449 | 496 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Gold 550 | 551 | 564 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
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| HCS CellMask Red cytoplasmic | 622 | 644 |
|
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| HiLyte Fluor™ 555 | 550 | 566 |
|
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| HiLyte Fluor™ 647 | 649 | 673 |
|
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| HiLyte Fluor™ 750 | 750 | 778 |
|
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| IRDye 800 CW-MeOH | 778 | 801 |
|
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| IRDye 800CW | 775 | 792 |
|
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| IRDye 800CW-NHS Ester | 775 | 792 |
|
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| IRDye 800Phosphoramidite | 257 | 809 |
|
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| IRDye 800RS | 787 | 812 |
|
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| KFP-Red | 575 | 599 |
|
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| LDS 751 | 560 | 711 |
|
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Near-IR Dead Cell Stain | 752 | 776 |
|
|
| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| LOLO-1 | 568 | 580 |
|
|
| LysoSensor Blue | 374 | 424 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| LysoTracker Green | 501 | 509 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Orange | 550 | 574 |
|
|
| mApple | 566 | 594 |
|
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| Marina Blue | 363 | 461 |
|
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| mCherry | 587 | 610 |
|
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| Merocyanine 540 | 559 | 579 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Deep Red | 641 | 662 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| MitoTracker™ Orange | 551 | 575 |
|
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| MitoTracker™ Red | 578 | 598 |
|
|
| mOrange | 546 | 562 |
|
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| mRFP | 555 | 583 |
|
|
| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
|
| mTFP1 | 462 | 492 |
|
|
| mWasabi | 493 | 509 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
|
| Nile red-triglyceride | 510 | 583 |
|
|
| OFP | 547 | 567 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
|
| Pacific Blue™ | 404 | 455 |
|
|
| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| PicoGreen® | 502 | 522 |
|
|
| PKH67 | 488 | 500 |
|
|
| POPO-3 | 533 | 574 |
|
|
| PO-PRO-3 | 539 | 570 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Pro-Q Diamond | 556 | 583 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Qdot® 800 Nanocrystals | 300 | 792 |
|
|
| Quasar® 705 | 688 | 706 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Red 650 | 649 | 665 |
|
|
| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine Green | 497 | 523 |
|
|
| Rhodamine phalloidin | 558 | 575 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| R-phycoerythrin (PE) | 565 | 576 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| Sodium Green | 507 | 532 |
|
|
| SpectrumBlue | 405 | 449 |
|
|
| SpectrumFRed (Far Red) | 650 | 673 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
|
| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
|
| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 59 | 622 | 644 |
|
|
| SYTO 60 | 650 | 681 |
|
|
| SYTO 61 | 618 | 650 |
|
|
| SYTO 82 | 540 | 559 |
|
|
| SYTO 9 | 483 | 500 |
|
|
| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| SYTOX Orange | 547 | 570 |
|
|
| SYTOX Red-DNA | 640 | 658 |
|
|
| TagBFP | 402 | 457 |
|
|
| TagCFP | 458 | 480 |
|
|