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BrightLine® full-multiband filter set, optimized for Cyan & Yellow Fluorescent Proteins and other like fluorophores, illuminated with Lumencor CELESTA light engine

Semrock Part Number: CELESTA-CFP/YFP-A-OMF

IDEX Part Number: S-416976  /

Semrock

This "full-multiband" filter set allows for simultaneous direct viewing or imaging of CFP (Cyan Fluorescent Protein) and YFP (Yellow Fluorescent Protein) (and other key fluorophores – see complete list above). This set has been optimized to be illuminated with Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths. This set has been designed to deliver significant fluorescence signal improvements for the CELESTA light engine compared with sets designed for standard broadband light sources such as mercury or xenon arc lamps or other LED-based light engines. The complete set is comprised of a dual-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.

  • Price Not Available.
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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,974.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
Aqua 431 430 478
ATTO 425 436 484
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 520 516 538
ATTO 532 501 545
BD Horizon V500 410 500
BOBO™-1 461 484
BODIPY R6G 528 547
Calcein 494 514
Calcium Green-1 507 529
Cerulean 434 473
CFP (ECFP) 433 475
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dronpa 502 516
DY-415 418 470
DY-505-Phalloidin 503 530
DyLight 488 493 518
ECFP 435 475
Eosin 525 546
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Orange fluorescent microspheres 540 559
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
Gold 525 521 551
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
JO-JO-1, JO-PRO-1 530 544
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mBBr+GSH 394 490
mHoneyDew 478 562
mTFP1 462 492
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PicoGreen® 502 522
POPO-1 433 457
PO-PRO-1 433 456
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
Sodium Green 507 532
SpectrumAqua 434 481
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 45 451 485
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Blue 444 470
SYTOX Green-DNA 504 524
TagCFP 458 480
TO-PRO-1 515 531
TOTO-1 514 531
TurboYFP 525 538
Venus 516 528
VIC 530 550
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
YFP (yellow GFP) 513 530
ZsYellow1 529 539

OMF Holder/Cube

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm

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