This "full-multiband" filter set allows for simultaneous direct viewing or imaging of CFP (Cyan Fluorescent Protein) and YFP (Yellow Fluorescent Protein) (and other key fluorophores – see complete list above). This set has been optimized to be illuminated with Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths. This set has been designed to deliver significant fluorescence signal improvements for the CELESTA light engine compared with sets designed for standard broadband light sources such as mercury or xenon arc lamps or other LED-based light engines. The complete set is comprised of a dual-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,063.50 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-449/520-25
FL-411981 / 0
- Tavg > 90% 439 – 459 nm & Tavg > 90% 510 – 530 nm
- Center Wavelengths of 449 nm & 520 nm and GMBWs of 20 nm & 20 nm
- Optimized for Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths
FF01-484/561-25
FL-412124 / 0
- Tavg > 90% 473 – 495 nm & Tavg > 90% 546 – 576 nm
- Center Wavelengths of 484 nm & 561 nm and GMBWs of 22 nm & 30 nm
- Optimized for Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths
FF471/539-Di01-25x36
FL-412130 / 0
- Dichroic Beamsplitter with edges at 471 nm & 539 nm
- Ravg > 95% 439 - 459 nm & Ravg > 95% 510 - 530 nm
- Tavg > 93% 473 - 495 nm & Tavg > 93% 546 - 576 nm
- Optimized for Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
|
| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
|
| 6-carboxyrhodamine 6G | 525 | 555 |
|
|
| 6-JOE | 520 | 548 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Acridine Yellow | 470 | 550 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
|
| Alexa Fluor® 514 | 518 | 543 |
|
|
| Alexa Fluor® 532 | 534 | 553 |
|
|
| Aqua 431 | 430 | 478 |
|
|
| ATTO 425 | 436 | 484 |
|
|
| ATTO 465 | 453 | 507 |
|
|
| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| ATTO 520 | 516 | 538 |
|
|
| ATTO 532 | 501 | 545 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| BODIPY R6G | 528 | 547 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Green-1 | 507 | 529 |
|
|
| Cerulean | 434 | 473 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| CyQUANT GR-DNA | 502 | 523 |
|
|
| DAF-FM-NO | 495 | 519 |
|
|
| Dronpa | 502 | 516 |
|
|
| DY-415 | 418 | 470 |
|
|
| DY-505-Phalloidin | 503 | 530 |
|
|
| DyLight 488 | 493 | 518 |
|
|
| ECFP | 435 | 475 |
|
|
| Eosin | 525 | 546 |
|
|
| FITC (Fluorescein) | 495 | 519 |
|
|
| FlAsH-CCPFCC | 511 | 530 |
|
|
| Fluo-3 | 506 | 527 |
|
|
| Fluo-4 | 494 | 516 |
|
|
| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
|
| Fluoro-Emerald | 494 | 518 |
|
|
| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
|
|
| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 1-43 | 473 | 579 |
|
|
| Gold 525 | 521 | 551 |
|
|
| Green 496 | 496 | 520 |
|
|
| Green 500 | 501 | 524 |
|
|
| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
|
| HiLyte Fluor™ 488 | 497 | 526 |
|
|
| JO-JO-1, JO-PRO-1 | 530 | 544 |
|
|
| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
|
| LysoTracker Yellow HCK-123 | 488 | 565 |
|
|
| Magnesium Green | 507 | 531 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| mHoneyDew | 478 | 562 |
|
|
| mTFP1 | 462 | 492 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
|
| Oregon Green™ 514 | 513 | 532 |
|
|
| PicoGreen® | 502 | 522 |
|
|
| POPO-1 | 433 | 457 |
|
|
| PO-PRO-1 | 433 | 456 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 545 Nanocrystals | 300 | 543 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine 123 | 507 | 529 |
|
|
| Rhodamine Green | 497 | 523 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| Sodium Green | 507 | 532 |
|
|
| SpectrumAqua | 434 | 481 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
|
| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
|
| SYTO 11 | 506 | 525 |
|
|
| SYTO 45 | 451 | 485 |
|
|
| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Blue | 444 | 470 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| TagCFP | 458 | 480 |
|
|
| TO-PRO-1 | 515 | 531 |
|
|
| TOTO-1 | 514 | 531 |
|
|
| TurboYFP | 525 | 538 |
|
|
| Venus | 516 | 528 |
|
|
| VIC | 530 | 550 |
|
|
| Vybrant DyeCycle Green | 506 | 534 |
|
|
| Vybrant DyeCycle Orange | 519 | 563 |
|
|
| YFP (yellow GFP) | 513 | 530 |
|
|
| ZsYellow1 | 529 | 539 |
|
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-449/520-25
FL-411981 / 0
- Tavg > 90% 439 – 459 nm & Tavg > 90% 510 – 530 nm
- Center Wavelengths of 449 nm & 520 nm and GMBWs of 20 nm & 20 nm
- Optimized for Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths
FF01-484/561-25
FL-412124 / 0
- Tavg > 90% 473 – 495 nm & Tavg > 90% 546 – 576 nm
- Center Wavelengths of 484 nm & 561 nm and GMBWs of 22 nm & 30 nm
- Optimized for Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths
FF471/539-Di01-25x36
FL-412130 / 0
- Dichroic Beamsplitter with edges at 471 nm & 539 nm
- Ravg > 95% 439 - 459 nm & Ravg > 95% 510 - 530 nm
- Tavg > 93% 473 - 495 nm & Tavg > 93% 546 - 576 nm
- Optimized for Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
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FITC-Di01-Clin-25x36
FL-007186