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BrightLine® single-band filter set, optimized for Brilliant Violet 480 and other like fluorophores

Semrock Part Number: BV480-2432A-000

IDEX Part Number: S-000673  /

Semrock

This filter set is ideal for use with Brilliant™ Violet 480 and a variety of other blue fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio. Are you co-labeling with BUV395, BV421? This filter set is optimized to minimize crosstalk with BUV395 and BV421.

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Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMakerâ„¢ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

BV480-2432A

S-001192 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-001194 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000051 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000052 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000050 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-002210 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-001193 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000049 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-001197 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000053 / 0

Out of Stock Backordered In Stock

1 of 2

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Yellow 470 550
AmCyan1 458 489
Aqua 431 430 478
ATTO 425 436 484
ATTO 465 453 507
BD Horizon V500 410 500
BOBOâ„¢-1 461 484
BV480 - BD Horizon Brilliantâ„¢ Violet 480 440 479
Cerulean 434 473
CFP (ECFP) 433 475
DY-415 418 470
ECFP 435 475
evoglow-Bs1 449 496
LysoSensor Green 448 502
mBBr+GSH 394 490
mTFP1 462 492
POPO-1 433 457
PO-PRO-1 433 456
SpectrumAqua 434 481
SYTO 45 451 485
SYTOX Blue 444 470
TagCFP 458 480

Specifications

BV480-2432A

S-001192 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-001194 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000051 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000052 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000050 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-002210 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-001193 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000049 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-001197 / 0

Out of Stock Backordered In Stock

BV480-2432A

S-000053 / 0

Out of Stock Backordered In Stock

1 of 2

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.