BrightLine® single-band filter set, optimized for Brilliant™ Violet 421 and other like fluorophores

Semrock Part Number: BV421-3824A-OMF

IDEX Part Number: S-001189  /

Semrock

This filter set is ideal for use with Brilliant™ Violet 421 and a variety of other blue fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio. Are you co-labeling with BUV395, BV510? This filter set is optimized to minimize crosstalk with BUV395 and BV510.

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  • 1,584.00 USD Each
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FF01-389/38-25

FL-004063 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370.0 – 408.0 nm
  • Center Wavelength of 389 nm and GMBW of 38 nm
  • FWHM of 41.9 nm

FF01-433/24-25

FL-004589 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 421 – 445 nm
  • Center Wavelength of 433 nm and GMBW of 24 nm
  • FWHM of 27.9 nm

FF414-Di01-25x36

FL-007088 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 414 nm
  • Ravg > 98% 327 – 409 nm
  • Tavg > 93% 420 - 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
7-hydroxy-4-methylcoumarin 360 442
Alexa Fluor® 405 401 422
AMCA (Aminomethylcoumarin) 350 448
BD Horizon V450 406 449
BFP (EBFP) 380 440
BV421 - BD Horizon Brilliant™ Violet 421 407 421
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
DAPI 359 461
DyLight 405 398 420
HiLyte Fluor™ 405 403 428
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
LysoTracker Blue 373 422
Marina Blue 363 461
Pacific Blue™ 404 455
sgBFP™ 387 451
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

Related Products

FF01-389/38-25

FL-004063 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370.0 – 408.0 nm
  • Center Wavelength of 389 nm and GMBW of 38 nm
  • FWHM of 41.9 nm

FF01-433/24-25

FL-004589 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 421 – 445 nm
  • Center Wavelength of 433 nm and GMBW of 24 nm
  • FWHM of 27.9 nm

FF414-Di01-25x36

FL-007088 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 414 nm
  • Ravg > 98% 327 – 409 nm
  • Tavg > 93% 420 - 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm