This filter set is ideal for use with Brilliant™ Violet 421 and a variety of other blue fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio. Are you co-labeling with BUV395, BV510? This filter set is optimized to minimize crosstalk with BUV395 and BV510.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,659.43 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-389/38-25
FL-004063 / 0
- Tavg > 93% 370.0 – 408.0 nm
- Center Wavelength of 389 nm and GMBW of 38 nm
- FWHM of 41.9 nm
FF01-433/24-25
FL-004589 / 0
- Tavg > 93% 421 – 445 nm
- Center Wavelength of 433 nm and GMBW of 24 nm
- FWHM of 27.9 nm
FF414-Di01-25x36
FL-007088 / 0
- Dichroic Beamsplitter with edge at 414 nm
- Ravg > 98% 327 – 409 nm
- Tavg > 93% 420 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Alexa Fluor® 405 | 401 | 422 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| BV421 - BD Horizon Brilliant™ Violet 421 | 407 | 421 |
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| Calcofluor White | 351 | 444 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
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| DAPI | 359 | 461 |
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| DyLight 405 | 398 | 420 |
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| HiLyte Fluor™ 405 | 403 | 428 |
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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| LysoSensor Blue | 374 | 424 |
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| LysoTracker Blue | 373 | 422 |
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| Marina Blue | 363 | 461 |
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| Pacific Blue™ | 404 | 455 |
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| sgBFP™ | 387 | 451 |
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| SpectrumBlue | 405 | 449 |
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| TagBFP | 402 | 457 |
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| Vybrant DyeCycle Violet | 369 | 437 |
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total selected
Clear selection
Select all 3 products from all pages.
FF01-389/38-25
FL-004063 / 0
- Tavg > 93% 370.0 – 408.0 nm
- Center Wavelength of 389 nm and GMBW of 38 nm
- FWHM of 41.9 nm
FF01-433/24-25
FL-004589 / 0
- Tavg > 93% 421 – 445 nm
- Center Wavelength of 433 nm and GMBW of 24 nm
- FWHM of 27.9 nm
FF414-Di01-25x36
FL-007088 / 0
- Dichroic Beamsplitter with edge at 414 nm
- Ravg > 98% 327 – 409 nm
- Tavg > 93% 420 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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