BrightLine® single-band filter set, optimized for Brilliant Violet 421 and other like fluorophores

Semrock Part Number: BV421-3824A-LDMP-ZERO

IDEX Part Number: S-001183  /

Semrock

This filter set is ideal for use with Brilliant™ Violet 421 and a variety of other blue fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio. Are you co-labeling with BUV395, BV510? This filter set is optimized to minimize crosstalk with BUV395 and BV510.

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  • 1,693.00 USD Each
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FF01-389/38-25

FL-004063 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370.0 – 408.0 nm
  • Center Wavelength of 389 nm and GMBW of 38 nm
  • FWHM of 41.9 nm

FF01-433/24-25

FL-004589 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 421 – 445 nm
  • Center Wavelength of 433 nm and GMBW of 24 nm
  • FWHM of 27.9 nm

FF414-Di01-25x36

FL-007088 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 414 nm
  • Ravg > 98% 327 – 409 nm
  • Tavg > 93% 420 - 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
7-hydroxy-4-methylcoumarin 360 442
Alexa Fluor® 405 401 422
AMCA (Aminomethylcoumarin) 350 448
BD Horizon V450 406 449
BFP (EBFP) 380 440
BV421 - BD Horizon Brilliant™ Violet 421 407 421
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
DAPI 359 461
DyLight 405 398 420
HiLyte Fluor™ 405 403 428
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
LysoTracker Blue 373 422
Marina Blue 363 461
Pacific Blue™ 404 455
sgBFP™ 387 451
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

Related Products

FF01-389/38-25

FL-004063 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370.0 – 408.0 nm
  • Center Wavelength of 389 nm and GMBW of 38 nm
  • FWHM of 41.9 nm

FF01-433/24-25

FL-004589 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 421 – 445 nm
  • Center Wavelength of 433 nm and GMBW of 24 nm
  • FWHM of 27.9 nm

FF414-Di01-25x36

FL-007088 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 414 nm
  • Ravg > 98% 327 – 409 nm
  • Tavg > 93% 420 - 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.