This filter set is ideal for use with Brilliant™ Violet 421 and a variety of other blue fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio. Are you co-labeling with BUV395, BV510? This filter set is optimized to minimize crosstalk with BUV395 and BV510.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,623.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-389/38-25
FL-004063 / 0
- Tavg > 93% 370.0 – 408.0 nm
- Center Wavelength of 389 nm and GMBW of 38 nm
- FWHM of 41.9 nm
FF01-433/24-25
FL-004589 / 0
- Tavg > 93% 421 – 445 nm
- Center Wavelength of 433 nm and GMBW of 24 nm
- FWHM of 27.9 nm
FF414-Di01-25x36
FL-007088 / 0
- Dichroic Beamsplitter with edge at 414 nm
- Ravg > 98% 327 – 409 nm
- Tavg > 93% 420 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Alexa Fluor® 405 | 401 | 422 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| BV421 - BD Horizon Brilliant™ Violet 421 | 407 | 421 |
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| Calcofluor White | 351 | 444 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
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| DAPI | 359 | 461 |
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| DyLight 405 | 398 | 420 |
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| HiLyte Fluor™ 405 | 403 | 428 |
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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| LysoSensor Blue | 374 | 424 |
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| LysoTracker Blue | 373 | 422 |
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| Marina Blue | 363 | 461 |
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| Pacific Blue™ | 404 | 455 |
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| sgBFP™ | 387 | 451 |
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| SpectrumBlue | 405 | 449 |
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| TagBFP | 402 | 457 |
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| Vybrant DyeCycle Violet | 369 | 437 |
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total selected
Clear selection
Select all 3 products from all pages.
FF01-389/38-25
FL-004063 / 0
- Tavg > 93% 370.0 – 408.0 nm
- Center Wavelength of 389 nm and GMBW of 38 nm
- FWHM of 41.9 nm
FF01-433/24-25
FL-004589 / 0
- Tavg > 93% 421 – 445 nm
- Center Wavelength of 433 nm and GMBW of 24 nm
- FWHM of 27.9 nm
FF414-Di01-25x36
FL-007088 / 0
- Dichroic Beamsplitter with edge at 414 nm
- Ravg > 98% 327 – 409 nm
- Tavg > 93% 420 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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