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BrightLine® single-band filter set, optimized for Brilliant™ Violet 421 and other like fluorophores

Semrock Part Number: BV421-3824A-LDMK-ZERO

IDEX Part Number: S-000043  /

Semrock

This filter set is ideal for use with Brilliant™ Violet 421 and a variety of other blue fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio. Are you co-labeling with BUV395, BV510? This filter set is optimized to minimize crosstalk with BUV395 and BV510.

  • Price Not Available.
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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,623.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
7-hydroxy-4-methylcoumarin 360 442
Alexa Fluor® 405 401 422
AMCA (Aminomethylcoumarin) 350 448
BD Horizon V450 406 449
BFP (EBFP) 380 440
BV421 - BD Horizon Brilliant™ Violet 421 407 421
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
DAPI 359 461
DyLight 405 398 420
HiLyte Fluor™ 405 403 428
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
LysoTracker Blue 373 422
Marina Blue 363 461
Pacific Blue™ 404 455
sgBFP™ 387 451
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

LDMK Holder/Cube

This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm

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