BrightLine Basic single-band filter set, optimized for BFP and other like fluorophores

Semrock Part Number: BFP-B-Basic-000

IDEX Part Number: S-443948  /

Semrock

Our Brightline® Basic fluorescence filter set belongs to our essential performance group. These sets are suitable for various fluorescence applications ranging from detection to imaging. These filters feature a fundamental and basic design, offered at a competitive price point. They are designed with thin-film hard-coated technology, they ensure exceptional performance and reliability.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 550.45 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • 0.00
    0.00
  • Notify me when available
We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components

FFB01-390/18-25

FL-442764

Each
  • Tavg > 93% 381 – 399 nm
  • Center Wavelength of 390 nm and GMBW of 18 nm
  • FWHM of 21.9 nm

FL-442764

FFB01-461/62-25

FL-442766

Each
  • Tavg > 93% 430– 492 nm
  • Center Wavelength of 461 nm and GMBW of 62 nm
  • FWHM of 66.1 nm

FL-442766

FF415-Di01-25x36

FL-442765

Each
  • Dichroic Beamsplitter with edge at 415 nm
  • Ravg > 98% 350 – 402 nm
  • Tavg > 95% 428 – 1100 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-442765

Optical Specifications Value
Blocking BrightLine® Basic filters offer essential performance specifications. These sets are suitable for various fluorescence applications ranging from detection to imaging.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 80-50
Dichroic Orientation Reflective coating side should face toward light source.
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
Alexa Fluor® 350 343 441
Alexa Fluor® 405 401 422
AMCA (Aminomethylcoumarin) 350 448
ATTO 390 392 477
BD Horizon V450 406 449
BD Horizon V500 410 500
BFP (EBFP) 380 440
Cascade Blueâ„¢ 401 419
CellTrace calcein violet 400 452
DAPI 359 461
DEAC 432 472
DY-415 418 470
DyLight 405 398 420
ECFP 435 475
ER-Trackerâ„¢ Blue-White DPX 372 557
HiLyte Fluorâ„¢ 405 403 428
Hoechst 33258 352 455
Hoechst 33342 352 455
Hoechst 34580 392 440
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
Marina Blue 363 461
mBBr+GSH 394 490
Pacific Blueâ„¢ 404 455
sgBFPâ„¢ 387 451
Sirius 355 424
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.