This filter set is ideal for use with Blue Fluorescent Protein (BFP) and other UV-excited, blue-emission fluorophores, including DAPI. This hard-coated filter set is the best value on the market, delivering an optimal combination of high brightness and contrast.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,394.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-390/18-25
FL-004665 / 0
- Tavg > 90% 380.8 – 399.3 nm
- Center Wavelength of 390 nm and GMBW of 18.5 nm
- FWHM of 22.4 nm
FF01-460/60-25
FL-004673 / 0
- Tavg > 90% 430.2 – 489.9 nm
- Center Wavelength of 460 nm and GMBW of 59.7 nm
- FWHM of 64.2 nm
FF416-Di01-25x36
FL-007089 / 0
- Dichroic Beamsplitter with edge at 416 nm
- Ravg > 90% 360 – 407 nm
- Tavg > 90% 425 – 575 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| Alexa Fluor® 350 | 343 | 441 |
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| Alexa Fluor® 405 | 401 | 422 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| ATTO 390 | 392 | 477 |
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| BD Horizon V450 | 406 | 449 |
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| BD Horizon V500 | 410 | 500 |
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| BFP (EBFP) | 380 | 440 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
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| DAPI | 359 | 461 |
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| DEAC | 432 | 472 |
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| DY-415 | 418 | 470 |
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| DyLight 405 | 398 | 420 |
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| ECFP | 435 | 475 |
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| ER-Tracker™ Blue-White DPX | 372 | 557 |
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| HiLyte Fluor™ 405 | 403 | 428 |
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| Hoechst 33258 | 352 | 455 |
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| Hoechst 33342 | 352 | 455 |
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| Hoechst 34580 | 392 | 440 |
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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| LysoSensor Blue | 374 | 424 |
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| Marina Blue | 363 | 461 |
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| mBBr+GSH | 394 | 490 |
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| Pacific Blue™ | 404 | 455 |
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| sgBFP™ | 387 | 451 |
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| Sirius | 355 | 424 |
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| SpectrumBlue | 405 | 449 |
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| TagBFP | 402 | 457 |
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| Vybrant DyeCycle Violet | 369 | 437 |
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total selected
Clear selection
Select all 3 products from all pages.
FF01-390/18-25
FL-004665 / 0
- Tavg > 90% 380.8 – 399.3 nm
- Center Wavelength of 390 nm and GMBW of 18.5 nm
- FWHM of 22.4 nm
FF01-460/60-25
FL-004673 / 0
- Tavg > 90% 430.2 – 489.9 nm
- Center Wavelength of 460 nm and GMBW of 59.7 nm
- FWHM of 64.2 nm
FF416-Di01-25x36
FL-007089 / 0
- Dichroic Beamsplitter with edge at 416 nm
- Ravg > 90% 360 – 407 nm
- Tavg > 90% 425 – 575 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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NFD01-532-25x36
FL-009363